Silencing of NbECR encoding a putative enoyl-CoA reductase results in disorganized membrane structures and epidermal cell ablation in Nicotiana benthamiana

Jong A. Park, Tae Wuk Kim, Seong Ki Kim, Taek Kim Woo, Hyun Sook Pai

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The very long chain fatty acids (VLCFAs) are synthesized by the microsomal fatty acid elongation system in plants. We investigated cellular function of NbECR putatively encoding enoyl-CoA reductase that catalyzes the last step of VLCFA elongation in Nicotiana benthamiana. Virus-induced gene silencing of NbECR produced necrotic lesions with typical cell death symptoms in leaves. In the affected tissues, ablation of the epidermal cell layer preceded disintegration of the whole leaf cell layers, and disorganized cellular membrane structure was evident. The amount of VLCFAs was reduced in the NbECR VIGS lines, suggesting NbECR function in elongation of VLCFAs. The results demonstrate that NbECR encodes a putative enoyl-CoA reductase and that the NbECR activity is essential for membrane biogenesis in N. benthamiana.

Original languageEnglish
Pages (from-to)4459-4464
Number of pages6
JournalFEBS Letters
Volume579
Issue number20
DOIs
Publication statusPublished - 2005 Aug 15

Fingerprint

Cell Membrane Structures
Fatty Acid Desaturases
Membrane structures
Ablation
Tobacco
Fatty Acids
Elongation
Cells
Membranes
Disintegration
Gene Silencing
Cell death
Cellular Structures
Viruses
Cell Death
Genes
Tissue

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

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abstract = "The very long chain fatty acids (VLCFAs) are synthesized by the microsomal fatty acid elongation system in plants. We investigated cellular function of NbECR putatively encoding enoyl-CoA reductase that catalyzes the last step of VLCFA elongation in Nicotiana benthamiana. Virus-induced gene silencing of NbECR produced necrotic lesions with typical cell death symptoms in leaves. In the affected tissues, ablation of the epidermal cell layer preceded disintegration of the whole leaf cell layers, and disorganized cellular membrane structure was evident. The amount of VLCFAs was reduced in the NbECR VIGS lines, suggesting NbECR function in elongation of VLCFAs. The results demonstrate that NbECR encodes a putative enoyl-CoA reductase and that the NbECR activity is essential for membrane biogenesis in N. benthamiana.",
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Silencing of NbECR encoding a putative enoyl-CoA reductase results in disorganized membrane structures and epidermal cell ablation in Nicotiana benthamiana. / Park, Jong A.; Kim, Tae Wuk; Kim, Seong Ki; Woo, Taek Kim; Pai, Hyun Sook.

In: FEBS Letters, Vol. 579, No. 20, 15.08.2005, p. 4459-4464.

Research output: Contribution to journalArticle

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