Silencing of NbNAP1 encoding a plastidic SufB-like protein affects chloroplast development in Nicotiana benthamiana

Chang Sook Ahn, Jeong Hee Lee, Hyun Sook Pai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

It was previously shown that AtNAP1 is a plastidic SufB protein involved in Fe-S cluster assembly in Arabidopsis. In this study, we investigated the effects of depleting SufB protein from plant cells using virus-induced gene silencing (VIGS). VIGS of NbNAP1 encoding a Nicotiana benthamiana homolog of AtNAP1 resulted in a leaf yellowing phenotype. NbNAP1 was expressed ubiquitously in plant tissues with the highest level in roots. A GFP fusion protein of the N-terminal region (M1-V103) of NbNAP1 was targeted to chloroplasts. Depletion of NbNAP1 resulted in reduced numbers of chloroplasts of reduced size. Mitochondria also seemed to be affected. Despite the reduced number and size of the chloroplasts in the NbNAP1 VIGS lines, the expression of many nuclear genes encoding chloroplast-targeted proteins and chlorophyll biosynthesis genes remained unchanged.

Original languageEnglish
Pages (from-to)112-118
Number of pages7
JournalMolecules and cells
Volume20
Issue number1
Publication statusPublished - 2005 Dec 1

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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