Silver nanoparticle-induced degranulation observed with quantitative phase microscopy

Wenzhong Yang, Seungrag Lee, Jiyong Lee, Yoonsung Bae, Dugyoung Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Monitoring a degranulation process in a live mast cell is a quite important issue in immunology and pharmacology. Because the size of a granule is normally much smaller than the resolution limit of an optical microscope system, there is no direct real-time live cell imaging technique for observing degranulation processes except for fluorescence imaging techniques. In this research, we propose optical quantitative phase microscopy (QPM) as a new observation tool to study degranulation processes in a live mast cell without any fluorescence labeling. We measure the cell volumes and the cross sectional profiles (x-z plane) of an RBL-2H3 cell and a HeLa cell, before and after they are exposed to calcium ionophore A23187 and silver nanoparticles (AgNPs). We verify that the volume and the cross sectional line profile of the RBL-2H3 cell were changed significantly when it was exposed to A23187. When 50 μg/mL of AgNP is used instead of A23187, the measurements of cell volume and cross sectional profiles indicate that RBL-2H3 cells also follow degranulation processes. Degranulation processes for these cells are verified by monitoring the increase of intracellular calcium ([Ca 2+] i)and histamine with fluorescent methods.

Original languageEnglish
Article number045005
JournalJournal of Biomedical Optics
Volume15
Issue number4
DOIs
Publication statusPublished - 2010 Jul 1

Fingerprint

Silver
Calcium
Microscopic examination
Fluorescence
silver
Immunology
Ionophores
Nanoparticles
microscopy
Imaging techniques
nanoparticles
Calcium Ionophores
Monitoring
cells
Labeling
Histamine
Microscopes
imaging techniques
calcium
immunology

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering

Cite this

Yang, Wenzhong ; Lee, Seungrag ; Lee, Jiyong ; Bae, Yoonsung ; Kim, Dugyoung. / Silver nanoparticle-induced degranulation observed with quantitative phase microscopy. In: Journal of Biomedical Optics. 2010 ; Vol. 15, No. 4.
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Silver nanoparticle-induced degranulation observed with quantitative phase microscopy. / Yang, Wenzhong; Lee, Seungrag; Lee, Jiyong; Bae, Yoonsung; Kim, Dugyoung.

In: Journal of Biomedical Optics, Vol. 15, No. 4, 045005, 01.07.2010.

Research output: Contribution to journalArticle

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