Simple and efficient capture of EGFR-expressing tumor cells using magnetic nanoparticles

Seong Min Jo, Seung Hyun Noh, Zongwen Jin, Yongjun Lim, Jinwoo Cheon, Hak Sung Kim

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Capturing tumor cells has attracted the attention because such cells can be used for diagnosing the occurrence and progression of cancers. Here we present a simple and efficient capturing of tumor cells using anti-EGFR antibody-conjugated magnetic nanoparticles. EGFR is a well-established epithelial cancer-specific marker that is over-expressed in many types of tumor cells. The conjugation of an anti-EGFR antibody with magnetic nanoparticles through a PEG-linker was shown to be highly efficient for preventing the non-specific binding of cells and improving the stability of the resulting nanoparticles even at a high salt concentration. The use of EGFR-targetable nanoparticles resulted in about 57% capture efficiency in 20 min for two cancer cell lines, PC9 (lung cancer) and HeLa (cervical cancer). The survival rate of the captured cells was approximately 85%, and most of the cells were retrievable, providing options for a further analysis of captured cells such as a mutational investigation of EGFR-related cancers. The present approach can be used for capturing circulating tumor cells (CTCs) expressing EGFR and the subsequent diagnostic analysis of the captured cells.

Original languageEnglish
Pages (from-to)144-152
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume201
DOIs
Publication statusPublished - 2014 Oct 1

Bibliographical note

Funding Information:
This research was supported by the Korea Health 21C R&D Project ( A040041 ) of Ministry for Health, Welfare and Family Affairs , the Pioneer Research Center Program ( 2008-2000218 ) through the National Research Foundation of Korea funded by the Ministry of Science, ICT & Future Planning , and the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning ( NRF-2013M3A9D6076530 ).

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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