Simple and efficient capture of EGFR-expressing tumor cells using magnetic nanoparticles

Seong Min Jo, Seung Hyun Noh, Zongwen Jin, Yongjun Lim, Jinwoo Cheon, Hak Sung Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Capturing tumor cells has attracted the attention because such cells can be used for diagnosing the occurrence and progression of cancers. Here we present a simple and efficient capturing of tumor cells using anti-EGFR antibody-conjugated magnetic nanoparticles. EGFR is a well-established epithelial cancer-specific marker that is over-expressed in many types of tumor cells. The conjugation of an anti-EGFR antibody with magnetic nanoparticles through a PEG-linker was shown to be highly efficient for preventing the non-specific binding of cells and improving the stability of the resulting nanoparticles even at a high salt concentration. The use of EGFR-targetable nanoparticles resulted in about 57% capture efficiency in 20 min for two cancer cell lines, PC9 (lung cancer) and HeLa (cervical cancer). The survival rate of the captured cells was approximately 85%, and most of the cells were retrievable, providing options for a further analysis of captured cells such as a mutational investigation of EGFR-related cancers. The present approach can be used for capturing circulating tumor cells (CTCs) expressing EGFR and the subsequent diagnostic analysis of the captured cells.

Original languageEnglish
Pages (from-to)144-152
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume201
DOIs
Publication statusPublished - 2014 Oct 1

Fingerprint

Tumors
tumors
cancer
Cells
Nanoparticles
nanoparticles
cells
Antibodies
antibodies
Polyethylene glycols
conjugation
cultured cells
progressions
markers
lungs
Salts
occurrences
salts

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Jo, Seong Min ; Noh, Seung Hyun ; Jin, Zongwen ; Lim, Yongjun ; Cheon, Jinwoo ; Kim, Hak Sung. / Simple and efficient capture of EGFR-expressing tumor cells using magnetic nanoparticles. In: Sensors and Actuators, B: Chemical. 2014 ; Vol. 201. pp. 144-152.
@article{195c05c8389f4d168a4a9c2c881c1cc6,
title = "Simple and efficient capture of EGFR-expressing tumor cells using magnetic nanoparticles",
abstract = "Capturing tumor cells has attracted the attention because such cells can be used for diagnosing the occurrence and progression of cancers. Here we present a simple and efficient capturing of tumor cells using anti-EGFR antibody-conjugated magnetic nanoparticles. EGFR is a well-established epithelial cancer-specific marker that is over-expressed in many types of tumor cells. The conjugation of an anti-EGFR antibody with magnetic nanoparticles through a PEG-linker was shown to be highly efficient for preventing the non-specific binding of cells and improving the stability of the resulting nanoparticles even at a high salt concentration. The use of EGFR-targetable nanoparticles resulted in about 57{\%} capture efficiency in 20 min for two cancer cell lines, PC9 (lung cancer) and HeLa (cervical cancer). The survival rate of the captured cells was approximately 85{\%}, and most of the cells were retrievable, providing options for a further analysis of captured cells such as a mutational investigation of EGFR-related cancers. The present approach can be used for capturing circulating tumor cells (CTCs) expressing EGFR and the subsequent diagnostic analysis of the captured cells.",
author = "Jo, {Seong Min} and Noh, {Seung Hyun} and Zongwen Jin and Yongjun Lim and Jinwoo Cheon and Kim, {Hak Sung}",
year = "2014",
month = "10",
day = "1",
doi = "10.1016/j.snb.2014.05.016",
language = "English",
volume = "201",
pages = "144--152",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",

}

Simple and efficient capture of EGFR-expressing tumor cells using magnetic nanoparticles. / Jo, Seong Min; Noh, Seung Hyun; Jin, Zongwen; Lim, Yongjun; Cheon, Jinwoo; Kim, Hak Sung.

In: Sensors and Actuators, B: Chemical, Vol. 201, 01.10.2014, p. 144-152.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Simple and efficient capture of EGFR-expressing tumor cells using magnetic nanoparticles

AU - Jo, Seong Min

AU - Noh, Seung Hyun

AU - Jin, Zongwen

AU - Lim, Yongjun

AU - Cheon, Jinwoo

AU - Kim, Hak Sung

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Capturing tumor cells has attracted the attention because such cells can be used for diagnosing the occurrence and progression of cancers. Here we present a simple and efficient capturing of tumor cells using anti-EGFR antibody-conjugated magnetic nanoparticles. EGFR is a well-established epithelial cancer-specific marker that is over-expressed in many types of tumor cells. The conjugation of an anti-EGFR antibody with magnetic nanoparticles through a PEG-linker was shown to be highly efficient for preventing the non-specific binding of cells and improving the stability of the resulting nanoparticles even at a high salt concentration. The use of EGFR-targetable nanoparticles resulted in about 57% capture efficiency in 20 min for two cancer cell lines, PC9 (lung cancer) and HeLa (cervical cancer). The survival rate of the captured cells was approximately 85%, and most of the cells were retrievable, providing options for a further analysis of captured cells such as a mutational investigation of EGFR-related cancers. The present approach can be used for capturing circulating tumor cells (CTCs) expressing EGFR and the subsequent diagnostic analysis of the captured cells.

AB - Capturing tumor cells has attracted the attention because such cells can be used for diagnosing the occurrence and progression of cancers. Here we present a simple and efficient capturing of tumor cells using anti-EGFR antibody-conjugated magnetic nanoparticles. EGFR is a well-established epithelial cancer-specific marker that is over-expressed in many types of tumor cells. The conjugation of an anti-EGFR antibody with magnetic nanoparticles through a PEG-linker was shown to be highly efficient for preventing the non-specific binding of cells and improving the stability of the resulting nanoparticles even at a high salt concentration. The use of EGFR-targetable nanoparticles resulted in about 57% capture efficiency in 20 min for two cancer cell lines, PC9 (lung cancer) and HeLa (cervical cancer). The survival rate of the captured cells was approximately 85%, and most of the cells were retrievable, providing options for a further analysis of captured cells such as a mutational investigation of EGFR-related cancers. The present approach can be used for capturing circulating tumor cells (CTCs) expressing EGFR and the subsequent diagnostic analysis of the captured cells.

UR - http://www.scopus.com/inward/record.url?scp=84901634780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901634780&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2014.05.016

DO - 10.1016/j.snb.2014.05.016

M3 - Article

VL - 201

SP - 144

EP - 152

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

ER -