Simple and robust measurement of blood plasma lysophospholipids using liquid chromatography mass spectrometry

Dong Yoon Ji, Chang Wan Lee, Se Hee Park, Eun Jig Lee, Do Yup Lee

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2 Citations (Scopus)


Single analytical procedure including extraction, liquid chromatography, and mass spectrometric analysis was evaluated for the simultaneous measurement of lysophospholipids (LPLs). LPLs, particularly, lysophosphatidic acids (LPA) and sphingosine 1-phosphate (S1P) are lipid messengers ubiquitously found in various biological matrix. The molecular species mediate important physiological roles in association with many diseases (e.g. cancer, inflammation, and neurodegenerative disease), which emphasize the significance of the simple and reliable analytical method for biomarker discovery and molecular mechanistic understanding. Thus, we developed analytical method mainly focusing on, but not limited by those lipid species S1P and LPA using reverse phase liquid chromatography-tandem mass spectrometry (RPLC-ESI-MS-MS). Extraction method was modified based on Folch method with optimally minimal level of ionization additive (ammonium formate 10 mM and formic acid). Reverse-phase liquid-chromatography was applied for chromatographical separation in combination with negative ionization mode electrospray-coupled Orbitrap mass spectrometry. The method validation was performed on human blood plasma in a non-targeted lipid profiling manner with full-scan MS mode and data-dependent MS/MS. The proposed method presented good inter-assay precision for primary targets, S1P and LPA. Subsequent analysis of other types of LPLs identified a broad range of lysophosphatidylcholines (LPCs) and lysophosphatidyl-ethanolamines (LPEs).

Original languageEnglish
Pages (from-to)109-113
Number of pages5
JournalMass Spectrometry Letters
Issue number4
Publication statusPublished - 2017 Dec

Bibliographical note

Funding Information:
(NRF) funded by the Ministry of Science and ICT.

Funding Information:
This study was supported by grants (grant number: NRF- 2016R1C1B2007982 and NRF-2016M3A9B6902062 to D. Y. L.) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT.

Publisher Copyright:
© Korean Society for Mass Spectrometry.

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Spectroscopy


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