Simple microdilution test for detection of metallo-β-lactamase production in pseudomonas aeruginosa

Roberta Migliavacca, Jean Denis Docquier, Claudia Mugnaioli, Gianfranco Amicosante, Rossana Daturi, Kyungwon Lee, Gian Maria Rossolini, Laura Pagani

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

A microdilution test measuring imipenem MICs in the presence or absence of a mixture of EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18 VIM-and 4 IMP-type metallo-β-lactamase (MBL) producers. The chelator mixture reduced by fourfold or more the imipenem MICs for MBL producers, while a lower effect or no effect was usually observed with MBL nonproducers.

Original languageEnglish
Pages (from-to)4388-4390
Number of pages3
JournalJournal of Clinical Microbiology
Volume40
Issue number11
DOIs
Publication statusPublished - 2002 Nov 1

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Imipenem
Pseudomonas aeruginosa
Chelating Agents
Edetic Acid
4-(1H-imidazol-4-ylmethyl)piperidine
1,10-phenanthroline

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Migliavacca, Roberta ; Docquier, Jean Denis ; Mugnaioli, Claudia ; Amicosante, Gianfranco ; Daturi, Rossana ; Lee, Kyungwon ; Rossolini, Gian Maria ; Pagani, Laura. / Simple microdilution test for detection of metallo-β-lactamase production in pseudomonas aeruginosa. In: Journal of Clinical Microbiology. 2002 ; Vol. 40, No. 11. pp. 4388-4390.
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Migliavacca, R, Docquier, JD, Mugnaioli, C, Amicosante, G, Daturi, R, Lee, K, Rossolini, GM & Pagani, L 2002, 'Simple microdilution test for detection of metallo-β-lactamase production in pseudomonas aeruginosa', Journal of Clinical Microbiology, vol. 40, no. 11, pp. 4388-4390. https://doi.org/10.1128/JCM.40.11.4388-4390.2002

Simple microdilution test for detection of metallo-β-lactamase production in pseudomonas aeruginosa. / Migliavacca, Roberta; Docquier, Jean Denis; Mugnaioli, Claudia; Amicosante, Gianfranco; Daturi, Rossana; Lee, Kyungwon; Rossolini, Gian Maria; Pagani, Laura.

In: Journal of Clinical Microbiology, Vol. 40, No. 11, 01.11.2002, p. 4388-4390.

Research output: Contribution to journalArticle

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