Abstract
A simple one-step purification method, using expanded bed, ion-exchange chromatography, for the fractionation of nisin Z produced by Lactococcus lactis subsp. lactis A164 was developed. The highest dynamic binding capacity (0.92) of the adsorbent was obtained at a superficial velocity of 367cm h-1, resulting in approx. 2.7-fold bed expansion. The range of pH for the maximum adsorption was 3-4. The isocratic elution with 0.15 m NaCl led to approx. >90% recovery. Single-step purification of nisin Z from unclarified A164 culture broth resulted in 31-fold purification with a 90% yield.
| Original language | English |
|---|---|
| Pages (from-to) | 1341-1345 |
| Number of pages | 5 |
| Journal | Biotechnology Letters |
| Volume | 26 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - 2004 Sep 1 |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
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Cheigh, C. I., Kook, M. C., Kim, S. B., Hong, Y. H., & Pyun, Y. R. (2004). Simple one-step purification of nisin Z from unclarified culture broth of Lactococcus lactis subsp. lactis A164 using expanded bed ion exchange chromatography. Biotechnology Letters, 26(17), 1341-1345. https://doi.org/10.1023/B:BILE.0000045630.29494.45