Simple one-step purification of nisin Z from unclarified culture broth of Lactococcus lactis subsp. lactis A164 using expanded bed ion exchange chromatography

Chan Ick Cheigh, Moo Chang Kook, Seong Bo Kim, Young Ho Hong, Yu Ryang Pyun

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A simple one-step purification method, using expanded bed, ion-exchange chromatography, for the fractionation of nisin Z produced by Lactococcus lactis subsp. lactis A164 was developed. The highest dynamic binding capacity (0.92) of the adsorbent was obtained at a superficial velocity of 367cm h-1, resulting in approx. 2.7-fold bed expansion. The range of pH for the maximum adsorption was 3-4. The isocratic elution with 0.15 m NaCl led to approx. >90% recovery. Single-step purification of nisin Z from unclarified A164 culture broth resulted in 31-fold purification with a 90% yield.

Original languageEnglish
Pages (from-to)1341-1345
Number of pages5
JournalBiotechnology Letters
Volume26
Issue number17
DOIs
Publication statusPublished - 2004 Sep 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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