Simultaneous analysis of acylcarnitines using MALDI-TOF mass spectrometry based on a parylene matrix chip

Joo Yoon Noh, Moon Ju Kim, Tae Gyeong Yun, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

Abstract

Short and medium chain acylcarnitines have been used for the diagnosis of various fatty acid oxidation and organic acid disorders. This report presents a multiplex and quantitative analysis of acylcarnitines using MALDI-TOF MS based on a parylene matrix chip. The parylene matrix chip was fabricated by the deposition of a nanoporous film of parylene on an organic matrix array, which reduced the number of mass peaks from the organic matrix in the low m/z range. Quantitative analysis was possible using the parylene matrix chip because of the formation of nano-sized sample crystals on the nanoporous parylene film. Seven acylcarnitines were quantitatively analyzed using the chip; the method detection range included the cut-off values for metabolic disorders. The seven acylcarnitines of different concentrations were simultaneously detected using the parylene matrix chip and the interference from the mixed carnitines was estimated. Real l-carnitine (C0) samples were analyzed using serial dilution, and the recoveries were calculated by comparisons with a standard curve.

Original languageEnglish
Pages (from-to)5044-5053
Number of pages10
JournalAnalyst
Volume147
Issue number22
DOIs
Publication statusPublished - 2022 Sep 23

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea [Grant numbers: NRF-2020R1A2B5B01002187, NRF-2020R1A5A101913111 and NRF-2021R1A2C209370611].

Publisher Copyright:
© 2022 The Royal Society of Chemistry.

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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