Simultaneous analysis of seven 2-hydroxy fatty acids as tert-butyldimethylsilyl derivatives in plasma by gas chromatography-mass spectrometry

Chan Seo, Jaehwan Yoon, Yumie Rhee, Jong Jin Kim, Sang Jip Nam, Wonjae Lee, Gwang Lee, Sung Tae Yee, Man Jeong Paik

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An efficient method for the simultaneous analysis of seven 2-hydroxy fatty acids (2-HFAs) as tert-butyldimethylsilyl (TBDMS) derivative was developed by gas chromatography-mass spectrometry in selected ion monitoring mode. New mass spectral data on 2-hydroxycapric, 2-hydroxypalmitic, 2-hydroxystearic and 2-hydroxybehenic acids as di-TBDMS derivatives for hydroxyl and carboxyl groups were built. Under the optimal conditions, the present method showed a good correlation coefficient (r≥0.999) in the range of 0.01-0.5μg. The precision showed low relative standard deviation of <10%, and the accuracy (percentage relative error) varied from -5.2 to 0.3 for the seven 2-HFAs studied. Recovery rates of all 2-HFAs were ≥93.2% with good precision. When applied to normal human plasma, seven 2-HFAs were positively identified. Therefore, the present efficient method will be useful for simultaneous analysis of 2-HFAs in plasma.

Original languageEnglish
Pages (from-to)156-160
Number of pages5
JournalBiomedical Chromatography
Volume29
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

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Plasma Gases
Hydroxy Acids
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Fatty Acids
Derivatives
Plasma (human)
Hydroxyl Radical
Ions
Plasmas
Recovery
Monitoring

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Seo, Chan ; Yoon, Jaehwan ; Rhee, Yumie ; Kim, Jong Jin ; Nam, Sang Jip ; Lee, Wonjae ; Lee, Gwang ; Yee, Sung Tae ; Paik, Man Jeong. / Simultaneous analysis of seven 2-hydroxy fatty acids as tert-butyldimethylsilyl derivatives in plasma by gas chromatography-mass spectrometry. In: Biomedical Chromatography. 2015 ; Vol. 29, No. 1. pp. 156-160.
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abstract = "An efficient method for the simultaneous analysis of seven 2-hydroxy fatty acids (2-HFAs) as tert-butyldimethylsilyl (TBDMS) derivative was developed by gas chromatography-mass spectrometry in selected ion monitoring mode. New mass spectral data on 2-hydroxycapric, 2-hydroxypalmitic, 2-hydroxystearic and 2-hydroxybehenic acids as di-TBDMS derivatives for hydroxyl and carboxyl groups were built. Under the optimal conditions, the present method showed a good correlation coefficient (r≥0.999) in the range of 0.01-0.5μg. The precision showed low relative standard deviation of <10{\%}, and the accuracy (percentage relative error) varied from -5.2 to 0.3 for the seven 2-HFAs studied. Recovery rates of all 2-HFAs were ≥93.2{\%} with good precision. When applied to normal human plasma, seven 2-HFAs were positively identified. Therefore, the present efficient method will be useful for simultaneous analysis of 2-HFAs in plasma.",
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Simultaneous analysis of seven 2-hydroxy fatty acids as tert-butyldimethylsilyl derivatives in plasma by gas chromatography-mass spectrometry. / Seo, Chan; Yoon, Jaehwan; Rhee, Yumie; Kim, Jong Jin; Nam, Sang Jip; Lee, Wonjae; Lee, Gwang; Yee, Sung Tae; Paik, Man Jeong.

In: Biomedical Chromatography, Vol. 29, No. 1, 01.01.2015, p. 156-160.

Research output: Contribution to journalArticle

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AU - Seo, Chan

AU - Yoon, Jaehwan

AU - Rhee, Yumie

AU - Kim, Jong Jin

AU - Nam, Sang Jip

AU - Lee, Wonjae

AU - Lee, Gwang

AU - Yee, Sung Tae

AU - Paik, Man Jeong

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