Simultaneous detection of multiple mutations in epidermal growth factor receptor based on fluorescence quenching of quantum dots

Taegyeong Kang, Hosub Lee, Dongcheol Choe, Sang Woo Joo, So Yeong Lee, Kyong Ah Yoon, Kangtaek Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have developed a simultaneous detection method for two common mutations in the epidermal growth factor receptor gene based on the fluorescence quenching phenomenon caused by aggregation of CdSe quantum dots. For detection of the in-frame deletion in exon 19 and the L858R point mutation in exon 21, water-soluble CdSe quantum dots with two sizes were functionalized using four different types of probe oligonucleotides. Addition of target oligonucleotides with the deletion mutation in exon 19 into the suspensions caused crosslinking-induced aggregation of green-emitting quantum dots, followed by the fluorescence quenching while that with the L858R point mutation resulted in aggregation of yellow-emitting quantum dots. In addition, targets with both deletion and point mutations caused aggregation of both green- and yellow-emitting quantum dots. This method allows a simultaneous detection of mutations in exon 19 and 21 of EGFR gene in a single experiment. We found that minimum mutant concentration that could be detected by this method was as low as 2% for deletion mutation, and 5% for point mutation. PCR products of EGFR gene were also used to confirm that our method could be used to detect mutation in amplified DNA fragments.

Original languageEnglish
Pages (from-to)558-561
Number of pages4
JournalBiosensors and Bioelectronics
Volume31
Issue number1
DOIs
Publication statusPublished - 2012 Jan 15

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this