Simultaneous Determination and Stability Test of Two Phthalic Anhydride Derivatives, Senkyunolide A and Z-Ligustilide, in the Water Extract of Cnidium Rhizome from Different Geographical Regions and Species Using HPLC-UVD Analysis

Hyeon Seok Jang, Kyo Bin Kang, Guijae Yoo, Euna Kwon, Jun Won Yun, Byeong Cheol Kang, In Kee Hong, Seung Hyun Kim, Sang Hyun Sung, Hyun Jong Cho, Young Pyo Jang, Heejung Yang

Research output: Contribution to journalArticle

Abstract

Cnidium rhizome from Cnidium officinale Makino and Ligusticum chuanxiong Hort (Umbelliferae) is traditionally used to treat pain in East Asian countries. Senkyunolide A and Z-ligustilide are phthalic anhydride derivatives that are the bioactive components of cnidium rhizome and chemical markers of the two species; they are also susceptible to external stimuli. In this study, we developed a method to identify two chemical markers using high-performance liquid chromatography (HPLC) with an ultraviolet detector (UVD); the contents of senkyunolide A and Z-ligustilide in cnidium rhizome from different regions were quantified. The stabilities of the compounds were tested at two different temperatures over 12 months. An HPLC-UVD system was used to identify and quantify senkyunolide A and Z-ligustilide in 10 C. officinale samples and 6 L. chuanxiong samples from China and Korea. The long-term stability of the C. officinale samples in dark conditions at 4 and 25 °C was also tested. The calibration curves for the two chemical markers showed high linearity (R2 = 1.0000), the precision was high (relative standard deviation (RSD) <4.17%), and the recoveries were within the range of 95–105%. The contents were mostly independent of the geographical origin and species, and >10% of the compounds degraded after storage at 4 and 25 °C for 6 months. A method was developed to rapidly identify two chemical markers in cnidium rhizome with good linearity, precision, and accuracy using HPLC-UVD. These markers will be utilized for the quality control or evaluation of herbal preparations containing cnidium rhizome.

Original languageEnglish
Pages (from-to)784-788
Number of pages5
JournalBulletin of the Korean Chemical Society
Volume39
Issue number6
DOIs
Publication statusPublished - 2018 Jun

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Ultraviolet detectors
Geographical regions
High performance liquid chromatography
Derivatives
Water
Plant Preparations
Quality control
Calibration
3-N-butyl-4,5-dihydrophthalide
phthalic anhydride
ligustilide
Temperature

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Jang, Hyeon Seok ; Kang, Kyo Bin ; Yoo, Guijae ; Kwon, Euna ; Yun, Jun Won ; Kang, Byeong Cheol ; Hong, In Kee ; Kim, Seung Hyun ; Sung, Sang Hyun ; Cho, Hyun Jong ; Jang, Young Pyo ; Yang, Heejung. / Simultaneous Determination and Stability Test of Two Phthalic Anhydride Derivatives, Senkyunolide A and Z-Ligustilide, in the Water Extract of Cnidium Rhizome from Different Geographical Regions and Species Using HPLC-UVD Analysis. In: Bulletin of the Korean Chemical Society. 2018 ; Vol. 39, No. 6. pp. 784-788.
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abstract = "Cnidium rhizome from Cnidium officinale Makino and Ligusticum chuanxiong Hort (Umbelliferae) is traditionally used to treat pain in East Asian countries. Senkyunolide A and Z-ligustilide are phthalic anhydride derivatives that are the bioactive components of cnidium rhizome and chemical markers of the two species; they are also susceptible to external stimuli. In this study, we developed a method to identify two chemical markers using high-performance liquid chromatography (HPLC) with an ultraviolet detector (UVD); the contents of senkyunolide A and Z-ligustilide in cnidium rhizome from different regions were quantified. The stabilities of the compounds were tested at two different temperatures over 12 months. An HPLC-UVD system was used to identify and quantify senkyunolide A and Z-ligustilide in 10 C. officinale samples and 6 L. chuanxiong samples from China and Korea. The long-term stability of the C. officinale samples in dark conditions at 4 and 25 °C was also tested. The calibration curves for the two chemical markers showed high linearity (R2 = 1.0000), the precision was high (relative standard deviation (RSD) <4.17{\%}), and the recoveries were within the range of 95–105{\%}. The contents were mostly independent of the geographical origin and species, and >10{\%} of the compounds degraded after storage at 4 and 25 °C for 6 months. A method was developed to rapidly identify two chemical markers in cnidium rhizome with good linearity, precision, and accuracy using HPLC-UVD. These markers will be utilized for the quality control or evaluation of herbal preparations containing cnidium rhizome.",
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Simultaneous Determination and Stability Test of Two Phthalic Anhydride Derivatives, Senkyunolide A and Z-Ligustilide, in the Water Extract of Cnidium Rhizome from Different Geographical Regions and Species Using HPLC-UVD Analysis. / Jang, Hyeon Seok; Kang, Kyo Bin; Yoo, Guijae; Kwon, Euna; Yun, Jun Won; Kang, Byeong Cheol; Hong, In Kee; Kim, Seung Hyun; Sung, Sang Hyun; Cho, Hyun Jong; Jang, Young Pyo; Yang, Heejung.

In: Bulletin of the Korean Chemical Society, Vol. 39, No. 6, 06.2018, p. 784-788.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Simultaneous Determination and Stability Test of Two Phthalic Anhydride Derivatives, Senkyunolide A and Z-Ligustilide, in the Water Extract of Cnidium Rhizome from Different Geographical Regions and Species Using HPLC-UVD Analysis

AU - Jang, Hyeon Seok

AU - Kang, Kyo Bin

AU - Yoo, Guijae

AU - Kwon, Euna

AU - Yun, Jun Won

AU - Kang, Byeong Cheol

AU - Hong, In Kee

AU - Kim, Seung Hyun

AU - Sung, Sang Hyun

AU - Cho, Hyun Jong

AU - Jang, Young Pyo

AU - Yang, Heejung

PY - 2018/6

Y1 - 2018/6

N2 - Cnidium rhizome from Cnidium officinale Makino and Ligusticum chuanxiong Hort (Umbelliferae) is traditionally used to treat pain in East Asian countries. Senkyunolide A and Z-ligustilide are phthalic anhydride derivatives that are the bioactive components of cnidium rhizome and chemical markers of the two species; they are also susceptible to external stimuli. In this study, we developed a method to identify two chemical markers using high-performance liquid chromatography (HPLC) with an ultraviolet detector (UVD); the contents of senkyunolide A and Z-ligustilide in cnidium rhizome from different regions were quantified. The stabilities of the compounds were tested at two different temperatures over 12 months. An HPLC-UVD system was used to identify and quantify senkyunolide A and Z-ligustilide in 10 C. officinale samples and 6 L. chuanxiong samples from China and Korea. The long-term stability of the C. officinale samples in dark conditions at 4 and 25 °C was also tested. The calibration curves for the two chemical markers showed high linearity (R2 = 1.0000), the precision was high (relative standard deviation (RSD) <4.17%), and the recoveries were within the range of 95–105%. The contents were mostly independent of the geographical origin and species, and >10% of the compounds degraded after storage at 4 and 25 °C for 6 months. A method was developed to rapidly identify two chemical markers in cnidium rhizome with good linearity, precision, and accuracy using HPLC-UVD. These markers will be utilized for the quality control or evaluation of herbal preparations containing cnidium rhizome.

AB - Cnidium rhizome from Cnidium officinale Makino and Ligusticum chuanxiong Hort (Umbelliferae) is traditionally used to treat pain in East Asian countries. Senkyunolide A and Z-ligustilide are phthalic anhydride derivatives that are the bioactive components of cnidium rhizome and chemical markers of the two species; they are also susceptible to external stimuli. In this study, we developed a method to identify two chemical markers using high-performance liquid chromatography (HPLC) with an ultraviolet detector (UVD); the contents of senkyunolide A and Z-ligustilide in cnidium rhizome from different regions were quantified. The stabilities of the compounds were tested at two different temperatures over 12 months. An HPLC-UVD system was used to identify and quantify senkyunolide A and Z-ligustilide in 10 C. officinale samples and 6 L. chuanxiong samples from China and Korea. The long-term stability of the C. officinale samples in dark conditions at 4 and 25 °C was also tested. The calibration curves for the two chemical markers showed high linearity (R2 = 1.0000), the precision was high (relative standard deviation (RSD) <4.17%), and the recoveries were within the range of 95–105%. The contents were mostly independent of the geographical origin and species, and >10% of the compounds degraded after storage at 4 and 25 °C for 6 months. A method was developed to rapidly identify two chemical markers in cnidium rhizome with good linearity, precision, and accuracy using HPLC-UVD. These markers will be utilized for the quality control or evaluation of herbal preparations containing cnidium rhizome.

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