Simultaneous determination of alkaloids and flavonoids in HMC05 preparation by HPLC-DAD

Seung Hyun Kim, Eun Jung Choi, Ki Yong Lee, Sang Hyun Sung, Heung Mook Shin

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


High performance liquid chromatography with diode array detector (HPLC-DAD) was developed for simultaneous determination of hesperidin, coptisine, palmatine, and berberine in HMC05, a standardized extract of eight different herbs. This method was validated in terms of specificity, linearity (r2 > 0.9995), precision (<5.0% RSD), and recoveries (96.6-110.5%). The LOD of these compounds were ranged from 101.6 to 171.7 ng. In addition, these four compounds in HMC05 were identified or tentatively characterized using HPLC-DAD-electrospray mass spectrometry (HPLC-DAD-ESI-MS).

Original languageEnglish
Pages (from-to)2917-2926
Number of pages10
JournalJournal of Liquid Chromatography and Related Technologies
Issue number19
Publication statusPublished - 2008 Jan

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry


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