Simultaneous determination of alkaloids and flavonoids in HMC05 preparation by HPLC-DAD

Seung Hyun Kim, Eun Jung Choi, Ki Yong Lee, Sang Hyun Sung, Heung Mook Shin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

High performance liquid chromatography with diode array detector (HPLC-DAD) was developed for simultaneous determination of hesperidin, coptisine, palmatine, and berberine in HMC05, a standardized extract of eight different herbs. This method was validated in terms of specificity, linearity (r2 > 0.9995), precision (<5.0% RSD), and recoveries (96.6-110.5%). The LOD of these compounds were ranged from 101.6 to 171.7 ng. In addition, these four compounds in HMC05 were identified or tentatively characterized using HPLC-DAD-electrospray mass spectrometry (HPLC-DAD-ESI-MS).

Original languageEnglish
Pages (from-to)2917-2926
Number of pages10
JournalJournal of Liquid Chromatography and Related Technologies
Volume31
Issue number19
DOIs
Publication statusPublished - 2008 Jan 1

Fingerprint

High performance liquid chromatography
Alkaloids
Flavonoids
Diodes
High Pressure Liquid Chromatography
Detectors
Hesperidin
Berberine
Mass spectrometry
Mass Spectrometry
Recovery

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

Cite this

Kim, Seung Hyun ; Choi, Eun Jung ; Lee, Ki Yong ; Sung, Sang Hyun ; Shin, Heung Mook. / Simultaneous determination of alkaloids and flavonoids in HMC05 preparation by HPLC-DAD. In: Journal of Liquid Chromatography and Related Technologies. 2008 ; Vol. 31, No. 19. pp. 2917-2926.
@article{b9c936bf13154bb7bf6442ba8f598375,
title = "Simultaneous determination of alkaloids and flavonoids in HMC05 preparation by HPLC-DAD",
abstract = "High performance liquid chromatography with diode array detector (HPLC-DAD) was developed for simultaneous determination of hesperidin, coptisine, palmatine, and berberine in HMC05, a standardized extract of eight different herbs. This method was validated in terms of specificity, linearity (r2 > 0.9995), precision (<5.0{\%} RSD), and recoveries (96.6-110.5{\%}). The LOD of these compounds were ranged from 101.6 to 171.7 ng. In addition, these four compounds in HMC05 were identified or tentatively characterized using HPLC-DAD-electrospray mass spectrometry (HPLC-DAD-ESI-MS).",
author = "Kim, {Seung Hyun} and Choi, {Eun Jung} and Lee, {Ki Yong} and Sung, {Sang Hyun} and Shin, {Heung Mook}",
year = "2008",
month = "1",
day = "1",
doi = "10.1080/10826070802424501",
language = "English",
volume = "31",
pages = "2917--2926",
journal = "Journal of Liquid Chromatography and Related Technologies",
issn = "1082-6076",
publisher = "Taylor and Francis Ltd.",
number = "19",

}

Simultaneous determination of alkaloids and flavonoids in HMC05 preparation by HPLC-DAD. / Kim, Seung Hyun; Choi, Eun Jung; Lee, Ki Yong; Sung, Sang Hyun; Shin, Heung Mook.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 31, No. 19, 01.01.2008, p. 2917-2926.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Simultaneous determination of alkaloids and flavonoids in HMC05 preparation by HPLC-DAD

AU - Kim, Seung Hyun

AU - Choi, Eun Jung

AU - Lee, Ki Yong

AU - Sung, Sang Hyun

AU - Shin, Heung Mook

PY - 2008/1/1

Y1 - 2008/1/1

N2 - High performance liquid chromatography with diode array detector (HPLC-DAD) was developed for simultaneous determination of hesperidin, coptisine, palmatine, and berberine in HMC05, a standardized extract of eight different herbs. This method was validated in terms of specificity, linearity (r2 > 0.9995), precision (<5.0% RSD), and recoveries (96.6-110.5%). The LOD of these compounds were ranged from 101.6 to 171.7 ng. In addition, these four compounds in HMC05 were identified or tentatively characterized using HPLC-DAD-electrospray mass spectrometry (HPLC-DAD-ESI-MS).

AB - High performance liquid chromatography with diode array detector (HPLC-DAD) was developed for simultaneous determination of hesperidin, coptisine, palmatine, and berberine in HMC05, a standardized extract of eight different herbs. This method was validated in terms of specificity, linearity (r2 > 0.9995), precision (<5.0% RSD), and recoveries (96.6-110.5%). The LOD of these compounds were ranged from 101.6 to 171.7 ng. In addition, these four compounds in HMC05 were identified or tentatively characterized using HPLC-DAD-electrospray mass spectrometry (HPLC-DAD-ESI-MS).

UR - http://www.scopus.com/inward/record.url?scp=53249113452&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53249113452&partnerID=8YFLogxK

U2 - 10.1080/10826070802424501

DO - 10.1080/10826070802424501

M3 - Article

VL - 31

SP - 2917

EP - 2926

JO - Journal of Liquid Chromatography and Related Technologies

JF - Journal of Liquid Chromatography and Related Technologies

SN - 1082-6076

IS - 19

ER -