Simultaneous determination of Baicalin and glycyrrhizin in Eul-Ja-Tang by HPLC/DAD

Mi Kyeong Lee, Ki Yong Lee, Seung Hyun Kim, Junghyun Park, Jung Hee Cho, Mi Hyun Oh, Ju Hyun Baek, Hyo Jin Kim, Young Choong Kim, Sang Hyun Sung

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A high performance liquid chromatographic (HPLC) method for the simultaneous determination of marker constituents, baicalin and glycyrrhizin was established for the quality control of traditional herbal medicinal preparation, Eul-Ja-Tang (EJT). Separation and quantification were successfully achieved with a Waters XTerra RP18 column (5 μm, 4.6 mm I.D. × 150 mm) by gradient elution of a mixture of acetonitrile and water containing 0.03% phosphoric acid (pH 2.03) at a flow rate of 1.0 mL/min. The diode-array UV/VIS detector (DAD) was used for the detection and the wavelength for quantification was set at 250 nm. The presence of baicalin and glycyrrhizin in this decoction was ascertained by retention time, spiking with each authentic standard and UV spectrum. Both baicalin and glycyrrhizin showed good linearity (r2 > 0.999) in a relatively wide concentration ranges. The R.S.D. for intra-day and inter-day precision was less than 5% and the limits of detection (LOD) were about 30 ng. The mean recovery of each compound was 99.5 - 101.2% with R.S.D. values less than 4.0%. This method was successfully applied to the determination of contents of baicalin and glycyrrhizin in three commercial products of EJT, which resulted in the difference in the contents of these compounds. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial EJT products.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalNatural Product Sciences
Volume14
Issue number3
Publication statusPublished - 2008 Sep 1

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Glycyrrhizic Acid
Liquids
Quality Control
Quality control
Ultraviolet detectors
Plant Preparations
Water
Limit of Detection
Diodes
Flow rate
Recovery
Wavelength
baicalin

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Organic Chemistry

Cite this

Lee, M. K., Lee, K. Y., Kim, S. H., Park, J., Cho, J. H., Oh, M. H., ... Sung, S. H. (2008). Simultaneous determination of Baicalin and glycyrrhizin in Eul-Ja-Tang by HPLC/DAD. Natural Product Sciences, 14(3), 147-151.
Lee, Mi Kyeong ; Lee, Ki Yong ; Kim, Seung Hyun ; Park, Junghyun ; Cho, Jung Hee ; Oh, Mi Hyun ; Baek, Ju Hyun ; Kim, Hyo Jin ; Kim, Young Choong ; Sung, Sang Hyun. / Simultaneous determination of Baicalin and glycyrrhizin in Eul-Ja-Tang by HPLC/DAD. In: Natural Product Sciences. 2008 ; Vol. 14, No. 3. pp. 147-151.
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abstract = "A high performance liquid chromatographic (HPLC) method for the simultaneous determination of marker constituents, baicalin and glycyrrhizin was established for the quality control of traditional herbal medicinal preparation, Eul-Ja-Tang (EJT). Separation and quantification were successfully achieved with a Waters XTerra RP18 column (5 μm, 4.6 mm I.D. × 150 mm) by gradient elution of a mixture of acetonitrile and water containing 0.03{\%} phosphoric acid (pH 2.03) at a flow rate of 1.0 mL/min. The diode-array UV/VIS detector (DAD) was used for the detection and the wavelength for quantification was set at 250 nm. The presence of baicalin and glycyrrhizin in this decoction was ascertained by retention time, spiking with each authentic standard and UV spectrum. Both baicalin and glycyrrhizin showed good linearity (r2 > 0.999) in a relatively wide concentration ranges. The R.S.D. for intra-day and inter-day precision was less than 5{\%} and the limits of detection (LOD) were about 30 ng. The mean recovery of each compound was 99.5 - 101.2{\%} with R.S.D. values less than 4.0{\%}. This method was successfully applied to the determination of contents of baicalin and glycyrrhizin in three commercial products of EJT, which resulted in the difference in the contents of these compounds. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial EJT products.",
author = "Lee, {Mi Kyeong} and Lee, {Ki Yong} and Kim, {Seung Hyun} and Junghyun Park and Cho, {Jung Hee} and Oh, {Mi Hyun} and Baek, {Ju Hyun} and Kim, {Hyo Jin} and Kim, {Young Choong} and Sung, {Sang Hyun}",
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Lee, MK, Lee, KY, Kim, SH, Park, J, Cho, JH, Oh, MH, Baek, JH, Kim, HJ, Kim, YC & Sung, SH 2008, 'Simultaneous determination of Baicalin and glycyrrhizin in Eul-Ja-Tang by HPLC/DAD', Natural Product Sciences, vol. 14, no. 3, pp. 147-151.

Simultaneous determination of Baicalin and glycyrrhizin in Eul-Ja-Tang by HPLC/DAD. / Lee, Mi Kyeong; Lee, Ki Yong; Kim, Seung Hyun; Park, Junghyun; Cho, Jung Hee; Oh, Mi Hyun; Baek, Ju Hyun; Kim, Hyo Jin; Kim, Young Choong; Sung, Sang Hyun.

In: Natural Product Sciences, Vol. 14, No. 3, 01.09.2008, p. 147-151.

Research output: Contribution to journalArticle

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T1 - Simultaneous determination of Baicalin and glycyrrhizin in Eul-Ja-Tang by HPLC/DAD

AU - Lee, Mi Kyeong

AU - Lee, Ki Yong

AU - Kim, Seung Hyun

AU - Park, Junghyun

AU - Cho, Jung Hee

AU - Oh, Mi Hyun

AU - Baek, Ju Hyun

AU - Kim, Hyo Jin

AU - Kim, Young Choong

AU - Sung, Sang Hyun

PY - 2008/9/1

Y1 - 2008/9/1

N2 - A high performance liquid chromatographic (HPLC) method for the simultaneous determination of marker constituents, baicalin and glycyrrhizin was established for the quality control of traditional herbal medicinal preparation, Eul-Ja-Tang (EJT). Separation and quantification were successfully achieved with a Waters XTerra RP18 column (5 μm, 4.6 mm I.D. × 150 mm) by gradient elution of a mixture of acetonitrile and water containing 0.03% phosphoric acid (pH 2.03) at a flow rate of 1.0 mL/min. The diode-array UV/VIS detector (DAD) was used for the detection and the wavelength for quantification was set at 250 nm. The presence of baicalin and glycyrrhizin in this decoction was ascertained by retention time, spiking with each authentic standard and UV spectrum. Both baicalin and glycyrrhizin showed good linearity (r2 > 0.999) in a relatively wide concentration ranges. The R.S.D. for intra-day and inter-day precision was less than 5% and the limits of detection (LOD) were about 30 ng. The mean recovery of each compound was 99.5 - 101.2% with R.S.D. values less than 4.0%. This method was successfully applied to the determination of contents of baicalin and glycyrrhizin in three commercial products of EJT, which resulted in the difference in the contents of these compounds. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial EJT products.

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