Simultaneous determination of bioactive phenolic compounds in the stem extract of Rhus verniciflua stokes by high performance liquid chromatography

Sun A. Kim, Seung Hyun Kim, In Sook Kim, Dongho Lee, Mi Sook Dong, Chun Soo Na, Nguyen Xuan Nhiem, Hye Hyun Yoo

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A simple, sensitive, and precise reversed-phase liquid chromatographic method was developed for the quantitative determination of 4 bioactive phenolic compounds (gallic acid, fustin, fisetin, and sulfuretin) from the stem extract of Rhus verniciflua stokes. Chromatographic analysis was performed on a Capcell Pak C18 column (150 × 4.6 mm, 3 μm) with a mobile phase consisting of 0.1% formic acid and 90% acetonitrile at a flow rate of 1 mL/min. Quantitation was performed using a UV-vis detector at 260 nm. The method was validated in terms of selectivity, linearity, accuracy, precision, and recovery. Excellent linear behavior was observed over the investigated concentration range (10-500 μ/mL for gallic acid, fustin, and fisetin; 0.5-100 μ/mL for sulfuretin) with correlation coefficient (r2) values >0.99. The intra- and inter-day precision over the concentration range of compounds was less than 6.65% (relative standard deviation) and the accuracy was between 92.42% and 103.62%. The mean recoveries for all the analytes were more than 92.18%. This method was successfully applied for the analysis of bioactive phenolic compounds in the R. verniciflua extracts.

Original languageEnglish
Pages (from-to)3813-3819
Number of pages7
JournalFood Chemistry
Volume141
Issue number4
DOIs
Publication statusPublished - 2013 Jan 1

Fingerprint

Toxicodendron vernicifluum
Rhus
Gallic Acid
formic acid
High performance liquid chromatography
phenolic compounds
high performance liquid chromatography
High Pressure Liquid Chromatography
Chromatographic analysis
Ultraviolet detectors
gallic acid
Recovery
stems
extracts
Flow rate
detectors
Chromatography
chromatography
Liquids
methodology

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science

Cite this

Kim, Sun A. ; Kim, Seung Hyun ; Kim, In Sook ; Lee, Dongho ; Dong, Mi Sook ; Na, Chun Soo ; Nhiem, Nguyen Xuan ; Yoo, Hye Hyun. / Simultaneous determination of bioactive phenolic compounds in the stem extract of Rhus verniciflua stokes by high performance liquid chromatography. In: Food Chemistry. 2013 ; Vol. 141, No. 4. pp. 3813-3819.
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Simultaneous determination of bioactive phenolic compounds in the stem extract of Rhus verniciflua stokes by high performance liquid chromatography. / Kim, Sun A.; Kim, Seung Hyun; Kim, In Sook; Lee, Dongho; Dong, Mi Sook; Na, Chun Soo; Nhiem, Nguyen Xuan; Yoo, Hye Hyun.

In: Food Chemistry, Vol. 141, No. 4, 01.01.2013, p. 3813-3819.

Research output: Contribution to journalArticle

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AU - Kim, Seung Hyun

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AU - Na, Chun Soo

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