Simultaneous determination of five active constituents in the aerial parts of Saururus chinensis by HPLC-DAD

Seung Hyun Kim; Eun Jung Choi; Mi Kyeong Lee; Ki Yong Lee; Jung Hyun Park; Young Choong Kim; Sang Hyun Sung

doi:10.1080/10826070903320426

Simultaneous determination of five active constituents in the aerial parts of Saururus chinensis by HPLC-DAD

Seung Hyun Kim, Eun Jung Choi, Mi Kyeong Lee, Ki Yong Lee, Jung Hyun Park, Young Choong Kim, Sang Hyun Sung

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The chemical profile of five main bioactive constituents including rutin, quercetin 3-O - D-glucuronopyranoside, quercetin 3-O - D-glucopyranosyl(12)-O - L-rhamnopyranoside, quercitrin and sauchinone in extract of Saururus chinensis was studied using high performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS). The HPLC-DAD conditions were optimized for the simultaneous analysis of these five compounds. This method was validated in terms of specificity, linearity (R20.9987), precision (6.4% RSD), and recoveries (93.9-103.0%). The limits of detection of these compounds were ranged from 38.7 to 648.4ng. In addition, the seasonal variation of the chemical composition of these main compounds in S. chinensis was investigated using this validated method, which resulted in a significant difference in the contents of these compounds according to the harvest time.

Original language	English
Pages (from-to)	2943-2953
Number of pages	11
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	32
Issue number	20
DOIs	https://doi.org/10.1080/10826070903320426
Publication status	Published - 2009 Dec

Bibliographical note

Funding Information:
This research was supported by a grant (10524) from Seoul R&BD Program, the Republic of Korea, and a grant PF06200-00 from Plant Diversity Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Korea.

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Pharmaceutical Science
Clinical Biochemistry

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title = "Simultaneous determination of five active constituents in the aerial parts of Saururus chinensis by HPLC-DAD",

abstract = "The chemical profile of five main bioactive constituents including rutin, quercetin 3-O - D-glucuronopyranoside, quercetin 3-O - D-glucopyranosyl(12)-O - L-rhamnopyranoside, quercitrin and sauchinone in extract of Saururus chinensis was studied using high performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS). The HPLC-DAD conditions were optimized for the simultaneous analysis of these five compounds. This method was validated in terms of specificity, linearity (R20.9987), precision (6.4% RSD), and recoveries (93.9-103.0%). The limits of detection of these compounds were ranged from 38.7 to 648.4ng. In addition, the seasonal variation of the chemical composition of these main compounds in S. chinensis was investigated using this validated method, which resulted in a significant difference in the contents of these compounds according to the harvest time.",

author = "Kim, {Seung Hyun} and Choi, {Eun Jung} and Lee, {Mi Kyeong} and Lee, {Ki Yong} and Park, {Jung Hyun} and Kim, {Young Choong} and Sung, {Sang Hyun}",

note = "Funding Information: This research was supported by a grant (10524) from Seoul R&BD Program, the Republic of Korea, and a grant PF06200-00 from Plant Diversity Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Korea.",

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AU - Park, Jung Hyun

AU - Kim, Young Choong

AU - Sung, Sang Hyun

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Simultaneous determination of five active constituents in the aerial parts of Saururus chinensis by HPLC-DAD

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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