Simultaneous determination of four active constituents in the roots of Scrophularia buergeriana by HPLC-DAD and LC-ESI-MS

Mi Kyeong Lee, Ok Gyung Choi, Jin Ho Park, Hi Jae Cho, Mi Jeong Ahn, Seung Hyun Kim, Young Choong Kim, Sang Hyun Sung

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The chemical profile of four main bioactive constituents including harpagoside (HS), 8-O-(E-p-methoxycinnamoyl)harpagide (HG), E-cinnamic acid (CA), and E-p-methoxycinnamic acid (MCA), in the extract of Scrophularia buergeriana was studied using HPLC-diode array detector-electrospray-MS (HPLC-DAD-ESI-MS). The HPLC-DAD conditions were optimized for the simultaneous analysis of these four compounds. This method was validated in terms of specificity, linearity (r2 >0.9998), precision (<2.0% RSD), and recoveries (94.4-115.1%). The LOD of these compounds were ranged from 5.9 to 37.8 ng. In addition, the main compounds in S. buergeriana and S. ningpoensis were quantified using this validated method, which resulted in significant difference in the contents of these compounds between the species.

Original languageEnglish
Pages (from-to)2345-2350
Number of pages6
JournalJournal of Separation Science
Volume30
Issue number15
DOIs
Publication statusPublished - 2007 Oct 1

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

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