Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes

A. E. Carruthers, T. K. Lake, L. Paterson, J. W. Allen, W. Sibbett, K. Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we describe methods to increase the functionality of conventional optical tweezer techniques through the use of a 410 nm laser diode to induce fluorescence in biological samples. For the first time, to our knowledge, we demonstrate the use of a violet diode laser to induce fluorescence in particles, which are simultaneously tweezed by an infrared laser. Using this technique we demonstrate the sorting of fluorescent polymer spheres and DAPI-stained chromosomes.

Original languageEnglish
Title of host publication2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003
Number of pages1
DOIs
Publication statusPublished - 2003 Dec 1
Event2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003 - Munich, Germany
Duration: 2003 Jun 222003 Jun 27

Publication series

NameConference on Lasers and Electro-Optics Europe - Technical Digest

Conference

Conference2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003
CountryGermany
CityMunich
Period03/6/2203/6/27

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials

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    Carruthers, A. E., Lake, T. K., Paterson, L., Allen, J. W., Sibbett, W., & Dholakia, K. (2003). Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes. In 2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003 [1313780] (Conference on Lasers and Electro-Optics Europe - Technical Digest). https://doi.org/10.1109/CLEOE.2003.1313780