Simultaneous quantification of oleins (triolein, diolein and monoolein) in mouse feces using liquid chromatography-electrospray ionization/mass spectrometry

Jong Hyun Lim, Jeongae Lee, Yura Jang, Hesson Chung, Wonyong Lee, Bong Chul Chung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fat malabsorption is an important cause of poor growth in infancy and childhood. Steatorrhea tests have been developed using various methods. Traditional measurements of stool fat, however, require large samples and it often takes as a week to complete the analysis. In this paper, a liquid chromatography-electrospray ionization/mass spectrometry (LC-ESI/MS) method was developed for simultaneous quantitative analysis of triacylglycerols, triolein, diolein and monoolein, in mouse feces. Moreover, the procedure was rapid, simple as well as compatible with LC-ESI/MS. Chloroform-isopropyl alcohol solution was used for fat-soluble sample extraction. After centrifugation and filtration, an analytical solution was prepared. Triolein, diolein and monoolein were separated using non-aqueous reversed-phase column with the mobile phase consisting of A (methanol) and B (acetone-isopropyl alcohol). The precision (% CV) and accuracy (% bias) of the assay were 3.8-14.7% and 85.2-114.9%, respectively. This method has been successfully applied to simultaneous determination of triolein, diolein and monoolein in feces from 30 mice. This method can therefore be applied to measure triacylglycerols in mouse feces accurately and precisely by LC-ESI/ MS, thereby helping to predictive biomarker in fat malabsorption and diagnostic research.

Original languageEnglish
Pages (from-to)68-73
Number of pages6
JournalMass Spectrometry Letters
Volume3
Issue number3
DOIs
Publication statusPublished - 2012 Dec 1

Fingerprint

Triolein
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Liquid chromatography
Feces
Liquid Chromatography
Mass spectrometry
Fats
2-Propanol
Triglycerides
Centrifugation
Steatorrhea
Biomarkers
Chloroform
Acetone
Methanol
Assays
diolein
monoolein
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Spectroscopy

Cite this

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title = "Simultaneous quantification of oleins (triolein, diolein and monoolein) in mouse feces using liquid chromatography-electrospray ionization/mass spectrometry",
abstract = "Fat malabsorption is an important cause of poor growth in infancy and childhood. Steatorrhea tests have been developed using various methods. Traditional measurements of stool fat, however, require large samples and it often takes as a week to complete the analysis. In this paper, a liquid chromatography-electrospray ionization/mass spectrometry (LC-ESI/MS) method was developed for simultaneous quantitative analysis of triacylglycerols, triolein, diolein and monoolein, in mouse feces. Moreover, the procedure was rapid, simple as well as compatible with LC-ESI/MS. Chloroform-isopropyl alcohol solution was used for fat-soluble sample extraction. After centrifugation and filtration, an analytical solution was prepared. Triolein, diolein and monoolein were separated using non-aqueous reversed-phase column with the mobile phase consisting of A (methanol) and B (acetone-isopropyl alcohol). The precision ({\%} CV) and accuracy ({\%} bias) of the assay were 3.8-14.7{\%} and 85.2-114.9{\%}, respectively. This method has been successfully applied to simultaneous determination of triolein, diolein and monoolein in feces from 30 mice. This method can therefore be applied to measure triacylglycerols in mouse feces accurately and precisely by LC-ESI/ MS, thereby helping to predictive biomarker in fat malabsorption and diagnostic research.",
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Simultaneous quantification of oleins (triolein, diolein and monoolein) in mouse feces using liquid chromatography-electrospray ionization/mass spectrometry. / Lim, Jong Hyun; Lee, Jeongae; Jang, Yura; Chung, Hesson; Lee, Wonyong; Chung, Bong Chul.

In: Mass Spectrometry Letters, Vol. 3, No. 3, 01.12.2012, p. 68-73.

Research output: Contribution to journalArticle

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AU - Lee, Wonyong

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