Abstract
This paper reports a single cell assay in compact disc (CD)-like lab chip with trapped single cells by centrifugal force. The main propose of this study was realization of cell assay on chip with the advantage of excluding the paracrine effect of adjacent cells. The channel in CD-like lab chip has many pitfalls at the outer side, in which cells were trapped respectively. The trap was able to fix each cell and the chemicals were treated by centrifugal pumping. Parameters that affects the trapping of a cell were the size of cells, the structure of micro-pattern, the magnitude of centrifugal force and the duration time. The trap ratio of cells was around 80% at optimal condition. Cytotoxicity and apoptosis test was done in CD and it was compared with conventional method to see the effect of single cell assay. Cytotoxicity was tested by the treatment of parformaldehyde and the apoptosis test was done by illumination UV light. The results of both assays indicated the overall trend of single assay in chip and conventional assay was same. However, apoptosis assay showed that the survival rate of single cell assay in chip was higher than that of conventional assay. Although it is still unclear that the result was caused by the effect of crowded cells, this single cell assay platform could be one of valuable tools in single cell assay.
| Original language | English |
|---|---|
| Title of host publication | Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007 |
| Pages | 557-560 |
| Number of pages | 4 |
| Publication status | Published - 2007 Dec 1 |
| Event | 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan Duration: 2007 Jan 21 → 2007 Jan 25 |
Publication series
| Name | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) |
|---|---|
| ISSN (Print) | 1084-6999 |
Other
| Other | 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 |
|---|---|
| Country | Japan |
| City | Kobe |
| Period | 07/1/21 → 07/1/25 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Mechanical Engineering
- Electrical and Electronic Engineering
Cite this
}
Single cell assay on CD-like lab chip using centrifugal single cell trap. / Lee, Sung Woo; Lee, In Hye; Ryu, Sung Shin; Kwak, Seung Min; Shin, Kyeong Sik; Kang, Ji Yoon; Jung, Hyo Il; Kim, Tae Song.
Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. 2007. p. 557-560 4433158 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Single cell assay on CD-like lab chip using centrifugal single cell trap
AU - Lee, Sung Woo
AU - Lee, In Hye
AU - Ryu, Sung Shin
AU - Kwak, Seung Min
AU - Shin, Kyeong Sik
AU - Kang, Ji Yoon
AU - Jung, Hyo Il
AU - Kim, Tae Song
PY - 2007/12/1
Y1 - 2007/12/1
N2 - This paper reports a single cell assay in compact disc (CD)-like lab chip with trapped single cells by centrifugal force. The main propose of this study was realization of cell assay on chip with the advantage of excluding the paracrine effect of adjacent cells. The channel in CD-like lab chip has many pitfalls at the outer side, in which cells were trapped respectively. The trap was able to fix each cell and the chemicals were treated by centrifugal pumping. Parameters that affects the trapping of a cell were the size of cells, the structure of micro-pattern, the magnitude of centrifugal force and the duration time. The trap ratio of cells was around 80% at optimal condition. Cytotoxicity and apoptosis test was done in CD and it was compared with conventional method to see the effect of single cell assay. Cytotoxicity was tested by the treatment of parformaldehyde and the apoptosis test was done by illumination UV light. The results of both assays indicated the overall trend of single assay in chip and conventional assay was same. However, apoptosis assay showed that the survival rate of single cell assay in chip was higher than that of conventional assay. Although it is still unclear that the result was caused by the effect of crowded cells, this single cell assay platform could be one of valuable tools in single cell assay.
AB - This paper reports a single cell assay in compact disc (CD)-like lab chip with trapped single cells by centrifugal force. The main propose of this study was realization of cell assay on chip with the advantage of excluding the paracrine effect of adjacent cells. The channel in CD-like lab chip has many pitfalls at the outer side, in which cells were trapped respectively. The trap was able to fix each cell and the chemicals were treated by centrifugal pumping. Parameters that affects the trapping of a cell were the size of cells, the structure of micro-pattern, the magnitude of centrifugal force and the duration time. The trap ratio of cells was around 80% at optimal condition. Cytotoxicity and apoptosis test was done in CD and it was compared with conventional method to see the effect of single cell assay. Cytotoxicity was tested by the treatment of parformaldehyde and the apoptosis test was done by illumination UV light. The results of both assays indicated the overall trend of single assay in chip and conventional assay was same. However, apoptosis assay showed that the survival rate of single cell assay in chip was higher than that of conventional assay. Although it is still unclear that the result was caused by the effect of crowded cells, this single cell assay platform could be one of valuable tools in single cell assay.
UR - http://www.scopus.com/inward/record.url?scp=52249088178&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=52249088178&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:52249088178
SN - 1424409519
SN - 9781424409518
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 557
EP - 560
BT - Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
ER -