Single cell assay on CD-like lab chip using centrifugal single cell trap

Sung Woo Lee, In Hye Lee, Sung Shin Ryu, Seung Min Kwak, Kyeong Sik Shin, Ji Yoon Kang, Hyo Il Jung, Tae Song Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper reports a single cell assay in compact disc (CD)-like lab chip with trapped single cells by centrifugal force. The main propose of this study was realization of cell assay on chip with the advantage of excluding the paracrine effect of adjacent cells. The channel in CD-like lab chip has many pitfalls at the outer side, in which cells were trapped respectively. The trap was able to fix each cell and the chemicals were treated by centrifugal pumping. Parameters that affects the trapping of a cell were the size of cells, the structure of micro-pattern, the magnitude of centrifugal force and the duration time. The trap ratio of cells was around 80% at optimal condition. Cytotoxicity and apoptosis test was done in CD and it was compared with conventional method to see the effect of single cell assay. Cytotoxicity was tested by the treatment of parformaldehyde and the apoptosis test was done by illumination UV light. The results of both assays indicated the overall trend of single assay in chip and conventional assay was same. However, apoptosis assay showed that the survival rate of single cell assay in chip was higher than that of conventional assay. Although it is still unclear that the result was caused by the effect of crowded cells, this single cell assay platform could be one of valuable tools in single cell assay.

Original languageEnglish
Title of host publicationProceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
Pages557-560
Number of pages4
Publication statusPublished - 2007 Dec 1
Event20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan
Duration: 2007 Jan 212007 Jan 25

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
CountryJapan
CityKobe
Period07/1/2107/1/25

Fingerprint

Assays
chips
traps
cells
Cell death
apoptosis
Cytotoxicity
Apoptosis
centrifugal force
Ultraviolet radiation
Lighting
fixing
pumping
platforms
illumination
trapping
trends

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Lee, S. W., Lee, I. H., Ryu, S. S., Kwak, S. M., Shin, K. S., Kang, J. Y., ... Kim, T. S. (2007). Single cell assay on CD-like lab chip using centrifugal single cell trap. In Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007 (pp. 557-560). [4433158] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
Lee, Sung Woo ; Lee, In Hye ; Ryu, Sung Shin ; Kwak, Seung Min ; Shin, Kyeong Sik ; Kang, Ji Yoon ; Jung, Hyo Il ; Kim, Tae Song. / Single cell assay on CD-like lab chip using centrifugal single cell trap. Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. 2007. pp. 557-560 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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title = "Single cell assay on CD-like lab chip using centrifugal single cell trap",
abstract = "This paper reports a single cell assay in compact disc (CD)-like lab chip with trapped single cells by centrifugal force. The main propose of this study was realization of cell assay on chip with the advantage of excluding the paracrine effect of adjacent cells. The channel in CD-like lab chip has many pitfalls at the outer side, in which cells were trapped respectively. The trap was able to fix each cell and the chemicals were treated by centrifugal pumping. Parameters that affects the trapping of a cell were the size of cells, the structure of micro-pattern, the magnitude of centrifugal force and the duration time. The trap ratio of cells was around 80{\%} at optimal condition. Cytotoxicity and apoptosis test was done in CD and it was compared with conventional method to see the effect of single cell assay. Cytotoxicity was tested by the treatment of parformaldehyde and the apoptosis test was done by illumination UV light. The results of both assays indicated the overall trend of single assay in chip and conventional assay was same. However, apoptosis assay showed that the survival rate of single cell assay in chip was higher than that of conventional assay. Although it is still unclear that the result was caused by the effect of crowded cells, this single cell assay platform could be one of valuable tools in single cell assay.",
author = "Lee, {Sung Woo} and Lee, {In Hye} and Ryu, {Sung Shin} and Kwak, {Seung Min} and Shin, {Kyeong Sik} and Kang, {Ji Yoon} and Jung, {Hyo Il} and Kim, {Tae Song}",
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Lee, SW, Lee, IH, Ryu, SS, Kwak, SM, Shin, KS, Kang, JY, Jung, HI & Kim, TS 2007, Single cell assay on CD-like lab chip using centrifugal single cell trap. in Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007., 4433158, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 557-560, 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007, Kobe, Japan, 07/1/21.

Single cell assay on CD-like lab chip using centrifugal single cell trap. / Lee, Sung Woo; Lee, In Hye; Ryu, Sung Shin; Kwak, Seung Min; Shin, Kyeong Sik; Kang, Ji Yoon; Jung, Hyo Il; Kim, Tae Song.

Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. 2007. p. 557-560 4433158 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Single cell assay on CD-like lab chip using centrifugal single cell trap

AU - Lee, Sung Woo

AU - Lee, In Hye

AU - Ryu, Sung Shin

AU - Kwak, Seung Min

AU - Shin, Kyeong Sik

AU - Kang, Ji Yoon

AU - Jung, Hyo Il

AU - Kim, Tae Song

PY - 2007/12/1

Y1 - 2007/12/1

N2 - This paper reports a single cell assay in compact disc (CD)-like lab chip with trapped single cells by centrifugal force. The main propose of this study was realization of cell assay on chip with the advantage of excluding the paracrine effect of adjacent cells. The channel in CD-like lab chip has many pitfalls at the outer side, in which cells were trapped respectively. The trap was able to fix each cell and the chemicals were treated by centrifugal pumping. Parameters that affects the trapping of a cell were the size of cells, the structure of micro-pattern, the magnitude of centrifugal force and the duration time. The trap ratio of cells was around 80% at optimal condition. Cytotoxicity and apoptosis test was done in CD and it was compared with conventional method to see the effect of single cell assay. Cytotoxicity was tested by the treatment of parformaldehyde and the apoptosis test was done by illumination UV light. The results of both assays indicated the overall trend of single assay in chip and conventional assay was same. However, apoptosis assay showed that the survival rate of single cell assay in chip was higher than that of conventional assay. Although it is still unclear that the result was caused by the effect of crowded cells, this single cell assay platform could be one of valuable tools in single cell assay.

AB - This paper reports a single cell assay in compact disc (CD)-like lab chip with trapped single cells by centrifugal force. The main propose of this study was realization of cell assay on chip with the advantage of excluding the paracrine effect of adjacent cells. The channel in CD-like lab chip has many pitfalls at the outer side, in which cells were trapped respectively. The trap was able to fix each cell and the chemicals were treated by centrifugal pumping. Parameters that affects the trapping of a cell were the size of cells, the structure of micro-pattern, the magnitude of centrifugal force and the duration time. The trap ratio of cells was around 80% at optimal condition. Cytotoxicity and apoptosis test was done in CD and it was compared with conventional method to see the effect of single cell assay. Cytotoxicity was tested by the treatment of parformaldehyde and the apoptosis test was done by illumination UV light. The results of both assays indicated the overall trend of single assay in chip and conventional assay was same. However, apoptosis assay showed that the survival rate of single cell assay in chip was higher than that of conventional assay. Although it is still unclear that the result was caused by the effect of crowded cells, this single cell assay platform could be one of valuable tools in single cell assay.

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M3 - Conference contribution

AN - SCOPUS:52249088178

SN - 1424409519

SN - 9781424409518

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 557

EP - 560

BT - Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007

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Lee SW, Lee IH, Ryu SS, Kwak SM, Shin KS, Kang JY et al. Single cell assay on CD-like lab chip using centrifugal single cell trap. In Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. 2007. p. 557-560. 4433158. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).