Single-cell cytokine profiling to investigate cellular functional diversity in hematopoietic malignancies

Jonathan J. Chen, Minsuk Kwak, Rong Fan

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Single-cell analysis of cytokine production is increasingly recognized as an important method to understand the inflammatory microenvironment and hematopoietic disease state. Certain cytokines are critical to the regulation of lineage specification, and the aberrant production of these cytokines can contribute to lineage reprogramming. Here, we describe of a platform combining subnanoliter microchambers and a highdensity antibody barcode array for the study of single-cell cytokine secretions in hematopoietic cancer cell populations. Key words Single-cell cytokine profiling, Immunophenotyping, Functional heterogeneity, Highthroughput multiplexed analysis, Cytokine secretions, Pathogenesis

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages243-254
Number of pages12
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1465
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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