Single-cell migration chip for chemotaxis-based microfluidic selection of heterogeneous cell populations

Yu Chih Chen, Steven G. Allen, Patrick N. Ingram, Ronald Buckanovich, Sofia D. Merajver, Euisik Yoon

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Tumor cell migration toward and intravasation into capillaries is an early and key event in cancer metastasis, yet not all cancer cells are imbued with the same capability to do so. This heterogeneity within a tumor is a fundamental property of cancer. Tools to help us understand what molecular characteristics allow a certain subpopulation of cells to spread from the primary tumor are thus critical for overcoming metastasis. Conventional in vitro migration platforms treat populations in aggregate, which leads to a masking of intrinsic differences among cells. Some migration assays reported recently have single-cell resolution, but these platforms do not provide for selective retrieval of the distinct migrating and non-migrating cell populations for further analysis. Thus, to study the intrinsic differences in cells responsible for chemotactic heterogeneity, we developed a single-cell migration platform so that individual cells' migration behavior can be studied and the heterogeneous population sorted based upon chemotactic phenotype. Furthermore, after migration, the highly chemotactic and non-chemotactic cells were retrieved and proved viable for later molecular analysis of their differences. Moreover, we modified the migration channel to resemble lymphatic capillaries to better understand how certain cancer cells are able to move through geometrically confining spaces.

Original languageEnglish
Article number9980
JournalScientific reports
Volume5
DOIs
Publication statusPublished - 2015 May 18

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'Single-cell migration chip for chemotaxis-based microfluidic selection of heterogeneous cell populations'. Together they form a unique fingerprint.

  • Cite this