Single cell migration chip using hydrodynamic cell positioning

Yu Chih Chen, Xia Lou, Patrick Ingram, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

We report a cell migration chip which can monitor chemotaxis at single cell resolution. The chip uses a cell capturing scheme based on the difference in hydrodynamic resistance between flow paths and cellular valving. A high capture rate over 94% is achieved by optimizing the geometry of capture sites and the length of serpentine structures. After capturing, cell migration experiments induced by chemotaxis were carried out using the fabricated platform, and the behavior of each single cell was successfully traced.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1409-1411
Number of pages3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Fingerprint

Hydrodynamics
Geometry
Experiments

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Chen, Y. C., Lou, X., Ingram, P., & Yoon, E. (2011). Single cell migration chip using hydrodynamic cell positioning. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1409-1411). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).
Chen, Yu Chih ; Lou, Xia ; Ingram, Patrick ; Yoon, Euisik. / Single cell migration chip using hydrodynamic cell positioning. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 1409-1411 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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abstract = "We report a cell migration chip which can monitor chemotaxis at single cell resolution. The chip uses a cell capturing scheme based on the difference in hydrodynamic resistance between flow paths and cellular valving. A high capture rate over 94{\%} is achieved by optimizing the geometry of capture sites and the length of serpentine structures. After capturing, cell migration experiments induced by chemotaxis were carried out using the fabricated platform, and the behavior of each single cell was successfully traced.",
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Chen, YC, Lou, X, Ingram, P & Yoon, E 2011, Single cell migration chip using hydrodynamic cell positioning. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 2, pp. 1409-1411, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11/10/2.

Single cell migration chip using hydrodynamic cell positioning. / Chen, Yu Chih; Lou, Xia; Ingram, Patrick; Yoon, Euisik.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1409-1411 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chen YC, Lou X, Ingram P, Yoon E. Single cell migration chip using hydrodynamic cell positioning. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1409-1411. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).