Single cell optical transfection

David J. Stevenson, Frank J. Gunn-Moore, Paul Campbell, Kishan Dholakia

Research output: Contribution to journalReview article

117 Citations (Scopus)

Abstract

The plasma membrane of a eukaryotic cell is impermeable to most hydrophilic substances, yet the insertion of these materials into cells is an extremely important and universal requirement for the cell biologist. To address this need, many transfection techniques have been developed including viral, lipoplex, polyplex, capillary microinjection, gene gun and electroporation. The current discussion explores a procedure called optical injection, where a laser field transiently increases the membrane permeability to allow species to be internalized. If the internalized substance is a nucleic acid, such as DNA, RNA or small interfering RNA (siRNA), then the process is called optical transfection. This contactless, aseptic, single cell transfection method provides a key nanosurgical tool to the microscopist-the intracellular delivery of reagents and single nanoscopic objects. The experimental possibilities enabled by this technology are only beginning to be realized. A review of optical transfection is presented, along with a forecast of future applications of this rapidly developing and exciting technology.

Original languageEnglish
Pages (from-to)863-871
Number of pages9
JournalJournal of the Royal Society Interface
Volume7
Issue number47
DOIs
Publication statusPublished - 2010 Jun 6

Fingerprint

RNA
Transfection
Nucleic acids
Cell membranes
Nucleic Acids
Small Interfering RNA
DNA
Genes
Technology
Membranes
Electroporation
Lasers
Firearms
Microinjections
Eukaryotic Cells
Permeability
Cell Membrane
Injections

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering

Cite this

Stevenson, David J. ; Gunn-Moore, Frank J. ; Campbell, Paul ; Dholakia, Kishan. / Single cell optical transfection. In: Journal of the Royal Society Interface. 2010 ; Vol. 7, No. 47. pp. 863-871.
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Stevenson, DJ, Gunn-Moore, FJ, Campbell, P & Dholakia, K 2010, 'Single cell optical transfection', Journal of the Royal Society Interface, vol. 7, no. 47, pp. 863-871. https://doi.org/10.1098/rsif.2009.0463

Single cell optical transfection. / Stevenson, David J.; Gunn-Moore, Frank J.; Campbell, Paul; Dholakia, Kishan.

In: Journal of the Royal Society Interface, Vol. 7, No. 47, 06.06.2010, p. 863-871.

Research output: Contribution to journalReview article

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