Single cell Proteolytic assays to investigate clonal heterogeneity and cell dynamics using efficient microfluidic cell loading

Yu Chih Chen, Yu Heng Cheng, Patrick Ingram, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Proteolytic degradation of extracellular matrix is critical in cancer invasion. In this work, we developed a microfluidic platform that provides high-efficiency cell loading and simple valveless isolation, allowing the characterization of proteolytic activities for a small sample (10-100 cells). Combined with a clonal sphere formation platform, we have successfully demonstrated the importance of microenvironmental cues for proteolytic activities and also investigated the differences among clones. Furthermore, the platform allows monitoring single-cell dynamics in proteolytic activity. The presented tool facilitates single-cell proteolytic analysis using small samples, and our findings illuminate the heterogeneous and dynamic nature of proteolytic activity.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages359-360
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016 Jan 1
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

Fingerprint

Microfluidics
Assays
Degradation
Monitoring

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Chen, Y. C., Cheng, Y. H., Ingram, P., & Yoon, E. (2016). Single cell Proteolytic assays to investigate clonal heterogeneity and cell dynamics using efficient microfluidic cell loading. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 359-360). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.
Chen, Yu Chih ; Cheng, Yu Heng ; Ingram, Patrick ; Yoon, Euisik. / Single cell Proteolytic assays to investigate clonal heterogeneity and cell dynamics using efficient microfluidic cell loading. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 359-360 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).
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abstract = "Proteolytic degradation of extracellular matrix is critical in cancer invasion. In this work, we developed a microfluidic platform that provides high-efficiency cell loading and simple valveless isolation, allowing the characterization of proteolytic activities for a small sample (10-100 cells). Combined with a clonal sphere formation platform, we have successfully demonstrated the importance of microenvironmental cues for proteolytic activities and also investigated the differences among clones. Furthermore, the platform allows monitoring single-cell dynamics in proteolytic activity. The presented tool facilitates single-cell proteolytic analysis using small samples, and our findings illuminate the heterogeneous and dynamic nature of proteolytic activity.",
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Chen, YC, Cheng, YH, Ingram, P & Yoon, E 2016, Single cell Proteolytic assays to investigate clonal heterogeneity and cell dynamics using efficient microfluidic cell loading. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 359-360, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16/10/9.

Single cell Proteolytic assays to investigate clonal heterogeneity and cell dynamics using efficient microfluidic cell loading. / Chen, Yu Chih; Cheng, Yu Heng; Ingram, Patrick; Yoon, Euisik.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 359-360 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - Proteolytic degradation of extracellular matrix is critical in cancer invasion. In this work, we developed a microfluidic platform that provides high-efficiency cell loading and simple valveless isolation, allowing the characterization of proteolytic activities for a small sample (10-100 cells). Combined with a clonal sphere formation platform, we have successfully demonstrated the importance of microenvironmental cues for proteolytic activities and also investigated the differences among clones. Furthermore, the platform allows monitoring single-cell dynamics in proteolytic activity. The presented tool facilitates single-cell proteolytic analysis using small samples, and our findings illuminate the heterogeneous and dynamic nature of proteolytic activity.

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Chen YC, Cheng YH, Ingram P, Yoon E. Single cell Proteolytic assays to investigate clonal heterogeneity and cell dynamics using efficient microfluidic cell loading. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 359-360. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).