Single-cell RNA-sequencing of migratory cancer cells sorted by microfluidics: Discovering drivers of cancer metastasis

Yu Chih Chen; Riley Brien; Saswat Sahoo; Woncheol Lee; Yu Heng Cheng; Seungwon Jung; Henry Haley; Kathryn Luker; Gary Luker; Euisik Yoon

Single-cell RNA-sequencing of migratory cancer cells sorted by microfluidics: Discovering drivers of cancer metastasis

Yu Chih Chen, Riley Brien, Saswat Sahoo, Woncheol Lee, Yu Heng Cheng, Seungwon Jung, Henry Haley, Kathryn Luker, Gary Luker, Euisik Yoon

Yonsei Advanced Science Institute

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Metastasis is the major cause of death in breast cancer patients, yet it remains challenging to pinpoint the key drivers in metastatic processes. We present a microfluidic cell migration platform enabling: (1) high-throughput migration experiments, (2) automatic image collection and analysis, and (3) reliable cell retrieval to enrich metastatic cancer cells. Using this platform, we have retrieved highly-migratory cells for in-vitro/in-vivo functional validation and single-cell whole transcriptome sequencing (RNA-Seq). In this manner, we identified new tumorigenic/metastatic biomarkers which were potential therapeutic targets.

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Publisher	Chemical and Biological Microsystems Society
Pages	130-131
Number of pages	2
ISBN (Electronic)	9781510897571
Publication status	Published - 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China Duration: 2018 Nov 11 → 2018 Nov 15

Publication series

Name	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume	1

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country	Taiwan, Province of China
City	Kaohsiung
Period	18/11/11 → 18/11/15

All Science Journal Classification (ASJC) codes

Chemistry(all)
Bioengineering
Chemical Engineering (miscellaneous)
Control and Systems Engineering

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Chen, Y. C., Brien, R., Sahoo, S., Lee, W., Cheng, Y. H., Jung, S., Haley, H., Luker, K., Luker, G., & Yoon, E. (2018). Single-cell RNA-sequencing of migratory cancer cells sorted by microfluidics: Discovering drivers of cancer metastasis. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 130-131). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 1). Chemical and Biological Microsystems Society.

Chen, Yu Chih ; Brien, Riley ; Sahoo, Saswat ; Lee, Woncheol ; Cheng, Yu Heng ; Jung, Seungwon ; Haley, Henry ; Luker, Kathryn ; Luker, Gary ; Yoon, Euisik. / Single-cell RNA-sequencing of migratory cancer cells sorted by microfluidics : Discovering drivers of cancer metastasis. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. pp. 130-131 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).

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title = "Single-cell RNA-sequencing of migratory cancer cells sorted by microfluidics: Discovering drivers of cancer metastasis",

abstract = "Metastasis is the major cause of death in breast cancer patients, yet it remains challenging to pinpoint the key drivers in metastatic processes. We present a microfluidic cell migration platform enabling: (1) high-throughput migration experiments, (2) automatic image collection and analysis, and (3) reliable cell retrieval to enrich metastatic cancer cells. Using this platform, we have retrieved highly-migratory cells for in-vitro/in-vivo functional validation and single-cell whole transcriptome sequencing (RNA-Seq). In this manner, we identified new tumorigenic/metastatic biomarkers which were potential therapeutic targets.",

author = "Chen, {Yu Chih} and Riley Brien and Saswat Sahoo and Woncheol Lee and Cheng, {Yu Heng} and Seungwon Jung and Henry Haley and Kathryn Luker and Gary Luker and Euisik Yoon",

note = "Publisher Copyright: Copyright{\textcopyright} (2018) by Chemical and Biological Microsystems Society.All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.; 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",

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language = "English",

series = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

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Chen, YC, Brien, R, Sahoo, S, Lee, W, Cheng, YH, Jung, S, Haley, H, Luker, K, Luker, G & Yoon, E 2018, Single-cell RNA-sequencing of migratory cancer cells sorted by microfluidics: Discovering drivers of cancer metastasis. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, vol. 1, Chemical and Biological Microsystems Society, pp. 130-131, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Kaohsiung, Taiwan, Province of China, 18/11/11.

Single-cell RNA-sequencing of migratory cancer cells sorted by microfluidics : Discovering drivers of cancer metastasis. / Chen, Yu Chih; Brien, Riley; Sahoo, Saswat; Lee, Woncheol; Cheng, Yu Heng; Jung, Seungwon; Haley, Henry; Luker, Kathryn; Luker, Gary; Yoon, Euisik.

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. p. 130-131 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Yoon, Euisik

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N2 - Metastasis is the major cause of death in breast cancer patients, yet it remains challenging to pinpoint the key drivers in metastatic processes. We present a microfluidic cell migration platform enabling: (1) high-throughput migration experiments, (2) automatic image collection and analysis, and (3) reliable cell retrieval to enrich metastatic cancer cells. Using this platform, we have retrieved highly-migratory cells for in-vitro/in-vivo functional validation and single-cell whole transcriptome sequencing (RNA-Seq). In this manner, we identified new tumorigenic/metastatic biomarkers which were potential therapeutic targets.

AB - Metastasis is the major cause of death in breast cancer patients, yet it remains challenging to pinpoint the key drivers in metastatic processes. We present a microfluidic cell migration platform enabling: (1) high-throughput migration experiments, (2) automatic image collection and analysis, and (3) reliable cell retrieval to enrich metastatic cancer cells. Using this platform, we have retrieved highly-migratory cells for in-vitro/in-vivo functional validation and single-cell whole transcriptome sequencing (RNA-Seq). In this manner, we identified new tumorigenic/metastatic biomarkers which were potential therapeutic targets.

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Chen YC, Brien R, Sahoo S, Lee W, Cheng YH, Jung S et al. Single-cell RNA-sequencing of migratory cancer cells sorted by microfluidics: Discovering drivers of cancer metastasis. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society. 2018. p. 130-131. (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).