Single cell suspension culture using polyhema coating for anoikis assay and sphere formation

Yu Chih Chen, Patrick Ingram, Xia Lou, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

In this work, we report a single cell suspension chip which can perform anoikis assay and sphere formation at single cell resolution. The proposed chip uses a hydrodynamic capturing scheme based 011 difference in flow resistance to guide cell loadings to an exact position in the array of microchambers. PolyHEMA coating is integrated with microfabrication to achieve a single cell suspension culture in each microchamber. We performed two sets of experiments: Anoikis assay of skov3 cells and sphere formation of SUM159 and MCF-7 cells; and the behavior of each single cell was successfully traced for more than ten days.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages106-108
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Fingerprint Dive into the research topics of 'Single cell suspension culture using polyhema coating for anoikis assay and sphere formation'. Together they form a unique fingerprint.

Cite this