Single-Cell Transcriptome Analysis of Colon Cancer Cell Response to 5-Fluorouracil-Induced DNA Damage

Sung Rye Park; Sim Namkoong; Leon Friesen; Chun Seok Cho; Zac Zezhi Zhang; Yu Chih Chen; Euisik Yoon; Chang H. Kim; Hojoong Kwak; Hyun Min Kang; Jun Hee Lee

doi:10.1016/j.celrep.2020.108077

Single-Cell Transcriptome Analysis of Colon Cancer Cell Response to 5-Fluorouracil-Induced DNA Damage

Sung Rye Park, Sim Namkoong, Leon Friesen, Chun Seok Cho, Zac Zezhi Zhang, Yu Chih Chen, Euisik Yoon, Chang H. Kim, Hojoong Kwak, Hyun Min Kang, Jun Hee Lee

Yonsei Advanced Science Institute

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Park et al. characterize transcriptome responses of colon cancer cells to 5FU-induced DNA damage at single-cell resolution. Many DNA damage response genes are heterogeneously expressed across the cell population. Specifically, cells undergoing different fates had distinct transcriptomic landscapes, characterized by the expression of fate-specific DNA damage response genes.

Original language	English
Article number	108077
Journal	Cell Reports
Volume	32
Issue number	8
DOIs	https://doi.org/10.1016/j.celrep.2020.108077
Publication status	Published - 2020 Aug 25

Bibliographical note

Funding Information:
We thank Dr. Y. Shah for cell lines; B. Gu, I.A. Semple, and B. Kim for technical assistance; Santa Cruz Biotechnology for antibodies; and Drs. M. Kim, Y. Xi, J. Connett, A.H. Kowalsky, and K.R. Spindler for their intellectual input and editorial assistance. This work was supported by the NIH ( R01DK102850 and R01DK114131 to J.H.L., U01HL137182 to H.M.K., and P30AG024824 , P30DK034933 , P30DK089503 , and P30CA046592 ), the Chan Zuckerberg Initiative (to H.M.K.), the MCubed Initiative (to J.H.L., H.M.K., and C.H.K.), the Organogenesis Fellowship (to S.N.), and an American Association for the Study of Liver Diseases pilot research award (to J.H.L. and H.M.K.).

Publisher Copyright:
© 2020 The Author(s)

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.celrep.2020.108077

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title = "Single-Cell Transcriptome Analysis of Colon Cancer Cell Response to 5-Fluorouracil-Induced DNA Damage",

abstract = "Park et al. characterize transcriptome responses of colon cancer cells to 5FU-induced DNA damage at single-cell resolution. Many DNA damage response genes are heterogeneously expressed across the cell population. Specifically, cells undergoing different fates had distinct transcriptomic landscapes, characterized by the expression of fate-specific DNA damage response genes.",

author = "Park, {Sung Rye} and Sim Namkoong and Leon Friesen and Cho, {Chun Seok} and Zhang, {Zac Zezhi} and Chen, {Yu Chih} and Euisik Yoon and Kim, {Chang H.} and Hojoong Kwak and Kang, {Hyun Min} and Lee, {Jun Hee}",

note = "Funding Information: We thank Dr. Y. Shah for cell lines; B. Gu, I.A. Semple, and B. Kim for technical assistance; Santa Cruz Biotechnology for antibodies; and Drs. M. Kim, Y. Xi, J. Connett, A.H. Kowalsky, and K.R. Spindler for their intellectual input and editorial assistance. This work was supported by the NIH ( R01DK102850 and R01DK114131 to J.H.L., U01HL137182 to H.M.K., and P30AG024824 , P30DK034933 , P30DK089503 , and P30CA046592 ), the Chan Zuckerberg Initiative (to H.M.K.), the MCubed Initiative (to J.H.L., H.M.K., and C.H.K.), the Organogenesis Fellowship (to S.N.), and an American Association for the Study of Liver Diseases pilot research award (to J.H.L. and H.M.K.). Publisher Copyright: {\textcopyright} 2020 The Author(s)",

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AU - Park, Sung Rye

AU - Namkoong, Sim

AU - Friesen, Leon

AU - Cho, Chun Seok

AU - Zhang, Zac Zezhi

AU - Chen, Yu Chih

AU - Yoon, Euisik

AU - Kim, Chang H.

AU - Kwak, Hojoong

AU - Kang, Hyun Min

AU - Lee, Jun Hee

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Single-Cell Transcriptome Analysis of Colon Cancer Cell Response to 5-Fluorouracil-Induced DNA Damage

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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