Single-Cell Transcriptome Analysis of Colon Cancer Cell Response to 5-Fluorouracil-Induced DNA Damage

Sung Rye Park, Sim Namkoong, Leon Friesen, Chun Seok Cho, Zac Zezhi Zhang, Yu Chih Chen, Euisik Yoon, Chang H. Kim, Hojoong Kwak, Hyun Min Kang, Jun Hee Lee

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19 Citations (Scopus)


Park et al. characterize transcriptome responses of colon cancer cells to 5FU-induced DNA damage at single-cell resolution. Many DNA damage response genes are heterogeneously expressed across the cell population. Specifically, cells undergoing different fates had distinct transcriptomic landscapes, characterized by the expression of fate-specific DNA damage response genes.

Original languageEnglish
Article number108077
JournalCell Reports
Issue number8
Publication statusPublished - 2020 Aug 25

Bibliographical note

Funding Information:
We thank Dr. Y. Shah for cell lines; B. Gu, I.A. Semple, and B. Kim for technical assistance; Santa Cruz Biotechnology for antibodies; and Drs. M. Kim, Y. Xi, J. Connett, A.H. Kowalsky, and K.R. Spindler for their intellectual input and editorial assistance. This work was supported by the NIH ( R01DK102850 and R01DK114131 to J.H.L., U01HL137182 to H.M.K., and P30AG024824 , P30DK034933 , P30DK089503 , and P30CA046592 ), the Chan Zuckerberg Initiative (to H.M.K.), the MCubed Initiative (to J.H.L., H.M.K., and C.H.K.), the Organogenesis Fellowship (to S.N.), and an American Association for the Study of Liver Diseases pilot research award (to J.H.L. and H.M.K.).

Publisher Copyright:
© 2020 The Author(s)

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)


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