Single-cell transcriptome analysis to study tumor-stromal interaction on cancer-stem-cells using vertical adhesion-suspension co-culture platforms

Yu Chih Chen, Zhixiong Zhang, Yu Heng Cheng, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The interaction between cancer-stem-cells and stromal cells is critical for development of tumor. In this work, we have developed a high-throughput (10,000-well), user-friendly, vertical co-culture platform for tumorsphere assays in interaction with cancer-associated-fibroblasts (CAFs). This platform has improved the throughput by more than two orders of magnitude. In addition, single-cell transcriptome analysis (mRNA Next Generation Sequencing (NGS)) was performed on the retrieved single cells, providing insights into the nature of functional CSCs.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages71-72
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016 Jan 1
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

Fingerprint

Stem cells
Cell culture
Tumors
Adhesion
Throughput
Fibroblasts
Assays
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Chen, Y. C., Zhang, Z., Cheng, Y. H., & Yoon, E. (2016). Single-cell transcriptome analysis to study tumor-stromal interaction on cancer-stem-cells using vertical adhesion-suspension co-culture platforms. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 71-72). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.
Chen, Yu Chih ; Zhang, Zhixiong ; Cheng, Yu Heng ; Yoon, Euisik. / Single-cell transcriptome analysis to study tumor-stromal interaction on cancer-stem-cells using vertical adhesion-suspension co-culture platforms. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 71-72 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).
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abstract = "The interaction between cancer-stem-cells and stromal cells is critical for development of tumor. In this work, we have developed a high-throughput (10,000-well), user-friendly, vertical co-culture platform for tumorsphere assays in interaction with cancer-associated-fibroblasts (CAFs). This platform has improved the throughput by more than two orders of magnitude. In addition, single-cell transcriptome analysis (mRNA Next Generation Sequencing (NGS)) was performed on the retrieved single cells, providing insights into the nature of functional CSCs.",
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year = "2016",
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series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
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Chen, YC, Zhang, Z, Cheng, YH & Yoon, E 2016, Single-cell transcriptome analysis to study tumor-stromal interaction on cancer-stem-cells using vertical adhesion-suspension co-culture platforms. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 71-72, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16/10/9.

Single-cell transcriptome analysis to study tumor-stromal interaction on cancer-stem-cells using vertical adhesion-suspension co-culture platforms. / Chen, Yu Chih; Zhang, Zhixiong; Cheng, Yu Heng; Yoon, Euisik.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 71-72 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chen YC, Zhang Z, Cheng YH, Yoon E. Single-cell transcriptome analysis to study tumor-stromal interaction on cancer-stem-cells using vertical adhesion-suspension co-culture platforms. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 71-72. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).