Single-channel microchip for fast screening and detailed identification of nitroaromatic explosives or organophosphate nerve agents

Joseph Wang, Martin Pumera, Madhu Prakash Chatrathi, Alberto Escarpa, Mustafa Musameh, Greg Collins, Ashok Mulchandani, Yuehe Lin, Khris Olsen

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

A single-channel chip-based analytical microsystem that allows rapid flow injection measurements of the total content of organic explosive or nerve agent compounds, as well as detailed micellar chromatographic identification of the individual ones, is described. The protocol involves repetitive rapid flow injection (screening) assays-to provide a timely warning and alarm-and switching to the separation (fingerprint identification) mode only when harmful compounds are detected. While micellar electrokinetic chromatography, in the presence of sodium dodecyl sulfate (SDS), is used for separating the neutral nitroaromatic explosive and nerve agent compounds, an operation without SDS leads to high-speed measurements of the "total" explosives or nerve agent content. Switching between the "flow injection" and "separation" modes is accomplished by rapidly exchanging the SDS-free and SDS-containing buffers in the separation channel. Amperometric detection was used for monitoring the separation. Key factors influencing the sample throughput, resolution, and sensitivity have been assessed and optimized. Assays rates of about 360 and 30/h can thus be realized for the "total" screening and "individual" measurements, respectively. Ultimately, such development will lead to the creation of a field-deployable microanalyzer and will enable transporting the forensic laboratory to the sample source.

Original languageEnglish
Pages (from-to)1187-1191
Number of pages5
JournalAnalytical Chemistry
Volume74
Issue number5
DOIs
Publication statusPublished - 2002 Mar 1

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Organophosphates
Sodium Dodecyl Sulfate
Screening
Assays
Microsystems
Chromatography
Buffers
Throughput
Nerve Agents
Monitoring

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Wang, Joseph ; Pumera, Martin ; Chatrathi, Madhu Prakash ; Escarpa, Alberto ; Musameh, Mustafa ; Collins, Greg ; Mulchandani, Ashok ; Lin, Yuehe ; Olsen, Khris. / Single-channel microchip for fast screening and detailed identification of nitroaromatic explosives or organophosphate nerve agents. In: Analytical Chemistry. 2002 ; Vol. 74, No. 5. pp. 1187-1191.
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Wang, J, Pumera, M, Chatrathi, MP, Escarpa, A, Musameh, M, Collins, G, Mulchandani, A, Lin, Y & Olsen, K 2002, 'Single-channel microchip for fast screening and detailed identification of nitroaromatic explosives or organophosphate nerve agents', Analytical Chemistry, vol. 74, no. 5, pp. 1187-1191. https://doi.org/10.1021/ac0111356

Single-channel microchip for fast screening and detailed identification of nitroaromatic explosives or organophosphate nerve agents. / Wang, Joseph; Pumera, Martin; Chatrathi, Madhu Prakash; Escarpa, Alberto; Musameh, Mustafa; Collins, Greg; Mulchandani, Ashok; Lin, Yuehe; Olsen, Khris.

In: Analytical Chemistry, Vol. 74, No. 5, 01.03.2002, p. 1187-1191.

Research output: Contribution to journalArticle

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AU - Olsen, Khris

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