Single-Droplet Multiplex Bioassay on a Robust and Stretchable Extreme Wetting Substrate through Vacuum-Based Droplet Manipulation

Heetak Han, Jung Seung Lee, Hyunchul Kim, Sera Shin, Jaehong Lee, Jongchan Kim, Xu Hou, Seung-Woo Cho, Jungmok Seo, Taeyoon Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Herein, a droplet manipulation system with a superamphiphobic (SPO)-superamphiphilic (SPI) patterned polydimethylsiloxane (PDMS) substrate is developed for a multiplex bioassay from single-droplet samples. The SPO substrate is fabricated by sequential spraying of adhesive and fluorinated silica nanoparticles onto a PDMS substrate. It is subsequently subjected to oxygen plasma with a patterned mask to form SPI patterns. The SPO layer exhibits extreme liquid repellency with a high contact angle (>150°) toward low surface tension and viscous biofluidic droplets (e.g., ethylene glycol, blood, dimethyl sulfoxide, and alginate hydrogel). In contrast, the SPI exhibits liquid adhesion with a near zero contact angle. Using the droplet manipulation system, various liquid droplets can be precisely manipulated and dispensed onto the predefined SPI patterns on the SPO PDMS substrate. This system enables a multiplex colorimetric bioassay, capable of detecting multiple analytes, including glucose, uric acid, and lactate, from a single sample droplet. In addition, the detection of glucose concentrations in a plasma droplet of diabetic and healthy mice are performed to demonstrate the feasibility of the proposed system for efficient clinical diagnostic applications.

Original languageEnglish
Pages (from-to)932-941
Number of pages10
JournalACS Nano
Volume12
Issue number2
DOIs
Publication statusPublished - 2018 Feb 27

Fingerprint

bioassay
Bioassay
wetting
Wetting
manipulators
Vacuum
vacuum
Substrates
glucose
Polydimethylsiloxane
liquids
uric acid
lactates
spraying
oxygen plasma
Contact angle
Glucose
adhesives
Liquids
blood

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Han, Heetak ; Lee, Jung Seung ; Kim, Hyunchul ; Shin, Sera ; Lee, Jaehong ; Kim, Jongchan ; Hou, Xu ; Cho, Seung-Woo ; Seo, Jungmok ; Lee, Taeyoon. / Single-Droplet Multiplex Bioassay on a Robust and Stretchable Extreme Wetting Substrate through Vacuum-Based Droplet Manipulation. In: ACS Nano. 2018 ; Vol. 12, No. 2. pp. 932-941.
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Single-Droplet Multiplex Bioassay on a Robust and Stretchable Extreme Wetting Substrate through Vacuum-Based Droplet Manipulation. / Han, Heetak; Lee, Jung Seung; Kim, Hyunchul; Shin, Sera; Lee, Jaehong; Kim, Jongchan; Hou, Xu; Cho, Seung-Woo; Seo, Jungmok; Lee, Taeyoon.

In: ACS Nano, Vol. 12, No. 2, 27.02.2018, p. 932-941.

Research output: Contribution to journalArticle

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AU - Lee, Jaehong

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AU - Hou, Xu

AU - Cho, Seung-Woo

AU - Seo, Jungmok

AU - Lee, Taeyoon

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