Abstract
We demonstrate single-shot quantitative phase imaging (QPI) in a platform of color-coded LED microscopy (cLEDscope). The light source in a conventional microscope is replaced by a circular LED pattern that is trisected into subregions with equal area, assigned to red, green, and blue colors. Image acquisition with a color image sensor and subsequent computation based on weak object transfer functions allow for the QPI of a transparent specimen. We also provide a correction method for color-leakage, which may be encountered in implementing our method with consumer-grade LEDs and image sensors. Most commercially available LEDs and image sensors do not provide spectrally isolated emissions and pixel responses, generating significant error in phase estimation in our method. We describe the correction scheme for this color-leakage issue, and demonstrate improved phase measurement accuracy. The computational model and single-exposure QPI capability of our method are presented by showing images of calibrated phase samples and cellular specimens.
| Original language | English |
|---|---|
| Pages (from-to) | 8398-8411 |
| Number of pages | 14 |
| Journal | Optics Express |
| Volume | 25 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2017 Apr 3 |
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All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics
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Single-exposure quantitative phase imaging in color-coded LED microscopy. / Lee, Wonchan; Jung, Daeseong; Ryu, Suho; Joo, Chulmin.
In: Optics Express, Vol. 25, No. 7, 03.04.2017, p. 8398-8411.Research output: Contribution to journal › Article
TY - JOUR
T1 - Single-exposure quantitative phase imaging in color-coded LED microscopy
AU - Lee, Wonchan
AU - Jung, Daeseong
AU - Ryu, Suho
AU - Joo, Chulmin
PY - 2017/4/3
Y1 - 2017/4/3
N2 - We demonstrate single-shot quantitative phase imaging (QPI) in a platform of color-coded LED microscopy (cLEDscope). The light source in a conventional microscope is replaced by a circular LED pattern that is trisected into subregions with equal area, assigned to red, green, and blue colors. Image acquisition with a color image sensor and subsequent computation based on weak object transfer functions allow for the QPI of a transparent specimen. We also provide a correction method for color-leakage, which may be encountered in implementing our method with consumer-grade LEDs and image sensors. Most commercially available LEDs and image sensors do not provide spectrally isolated emissions and pixel responses, generating significant error in phase estimation in our method. We describe the correction scheme for this color-leakage issue, and demonstrate improved phase measurement accuracy. The computational model and single-exposure QPI capability of our method are presented by showing images of calibrated phase samples and cellular specimens.
AB - We demonstrate single-shot quantitative phase imaging (QPI) in a platform of color-coded LED microscopy (cLEDscope). The light source in a conventional microscope is replaced by a circular LED pattern that is trisected into subregions with equal area, assigned to red, green, and blue colors. Image acquisition with a color image sensor and subsequent computation based on weak object transfer functions allow for the QPI of a transparent specimen. We also provide a correction method for color-leakage, which may be encountered in implementing our method with consumer-grade LEDs and image sensors. Most commercially available LEDs and image sensors do not provide spectrally isolated emissions and pixel responses, generating significant error in phase estimation in our method. We describe the correction scheme for this color-leakage issue, and demonstrate improved phase measurement accuracy. The computational model and single-exposure QPI capability of our method are presented by showing images of calibrated phase samples and cellular specimens.
UR - http://www.scopus.com/inward/record.url?scp=85016970595&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85016970595&partnerID=8YFLogxK
U2 - 10.1364/OE.25.008398
DO - 10.1364/OE.25.008398
M3 - Article
C2 - 28380952
AN - SCOPUS:85016970595
VL - 25
SP - 8398
EP - 8411
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 7
ER -