Single-stranded DNA binding factor AtWHY1 modulates telomere length homeostasis in Arabidopsis

Hyun Hee Yoo, Chian Kwon, Myeong Min Lee, In Kwon Chung

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Telomere homeostasis, a process that is essential for the maintenance of chromosome integrity, is regulated by telomerase and a collection of associated proteins. By mass spectrometry we have identified a new telomeric protein encoded by the AtWHY1 (Arabidopsis thaliana Whirly 1) gene in Arabidopsis. AtWHY1 specifically binds the single-stranded plant telomeric DNA sequences, but not double-stranded telomeric DNA. To gain insights into the function of AtWHY1 in telomere biogenesis, we have identified two Arabidopsis lines harboring T-DNA insertions in AtWHY1. These lines exhibit neither growth nor developmental defects. However, AtWHY1-deficient plants show a steady increase in the length of telomere tracts over generations. This telomere elongation is correlated with a significant increase in telomerase activity. On the contrary, transgenic plants expressing AtWHY1 show a decreased telomerase activity and shortened telomeres. The evidence presented here indicates that AtWHY1 is a new family of telomere end-binding proteins that plays a role in regulating telomere-length homeostasis in Arabidopsis.

Original languageEnglish
Pages (from-to)442-451
Number of pages10
JournalPlant Journal
Volume49
Issue number3
DOIs
Publication statusPublished - 2007 Feb 1

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Telomere Homeostasis
single-stranded DNA
Single-Stranded DNA
telomeres
Arabidopsis
homeostasis
Arabidopsis thaliana
telomerase
Telomerase
Telomere
Telomere-Binding Proteins
new family
Plant DNA
Arabidopsis Proteins
DNA
Telomere Shortening
Genetically Modified Plants
binding proteins
transgenic plants
proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

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abstract = "Telomere homeostasis, a process that is essential for the maintenance of chromosome integrity, is regulated by telomerase and a collection of associated proteins. By mass spectrometry we have identified a new telomeric protein encoded by the AtWHY1 (Arabidopsis thaliana Whirly 1) gene in Arabidopsis. AtWHY1 specifically binds the single-stranded plant telomeric DNA sequences, but not double-stranded telomeric DNA. To gain insights into the function of AtWHY1 in telomere biogenesis, we have identified two Arabidopsis lines harboring T-DNA insertions in AtWHY1. These lines exhibit neither growth nor developmental defects. However, AtWHY1-deficient plants show a steady increase in the length of telomere tracts over generations. This telomere elongation is correlated with a significant increase in telomerase activity. On the contrary, transgenic plants expressing AtWHY1 show a decreased telomerase activity and shortened telomeres. The evidence presented here indicates that AtWHY1 is a new family of telomere end-binding proteins that plays a role in regulating telomere-length homeostasis in Arabidopsis.",
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Single-stranded DNA binding factor AtWHY1 modulates telomere length homeostasis in Arabidopsis. / Yoo, Hyun Hee; Kwon, Chian; Lee, Myeong Min; Chung, In Kwon.

In: Plant Journal, Vol. 49, No. 3, 01.02.2007, p. 442-451.

Research output: Contribution to journalArticle

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