Stromal and immune cells in the tumor microenvironment (TME) have been shown to directly affect high-grade serous ovarian cancer (HGSC) malignant phenotypes, however, how these cells interact to influence HGSC patients’ survival remains largely unknown. To investigate the cell-cell communication in such a complex TME, we developed a SpatioImageOmics (SIO) pipeline that combines imaging mass cytometry (IMC), location-specific transcriptomics, and deep learning to identify the distribution of various stromal, tumor and immune cells as well as their spatial relationship in TME. The SIO pipeline automatically and accurately segments cells and extracts salient cellular features to identify biomarkers, and multiple nearest-neighbor interactions among tumor, immune, and stromal cells that coordinate to influence overall survival rates in HGSC patients. In addition, SIO integrates IMC data with microdissected tumor and stromal transcriptomes from the same patients to identify novel signaling networks, which would lead to the discovery of novel survival rate-modulating mechanisms in HGSC patients.
|Publication status||Published - 2021 Apr 2|
Bibliographical noteFunding Information:
Funding: This study was supported in part by W81XWH-17-1-0126 and W81XWH-16-1-0038 from the Ovarian Cancer Research Program, US Department of Defense; the MD Anderson Cancer Center Support Grant P30CA016672 from the National Institutes of Health; the Sister Institution Network Fund from the University of Texas MD Anderson Cancer Center; the US Department of Health and Human Services; the T.T. and W.F. Chao Foundation, Stephanie C. Stelter Foundation, John S. Dunn Research Foundation; and Carole Walter Looke Fund. Y.Z. was supported by a Computational Cancer Biology Training Program Fellowship from the Gulf Coast Consortia (CPRIT Grant No. RP170593) with S.C.M. and S.T.C.W. as mentors. This study was supported in part by the National Institutes of Health through M. D. Anderson’s Cancer Center Support Grant CA016672, the NCI’s Research Specialist 1 R50 CA243707-01A1, and a Shared Instrumentation Award from the Cancer Prevention Research Institution of Texas (CPRIT), RP121010.
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
All Science Journal Classification (ASJC) codes
- Cancer Research