Site specific multi-point fluorescence measurement system for fluorescence correlation spectroscopy

Woosub Song, Sucbei Moon, Byoung Cheol Lee, Haedeun Kim, Chul Seung Park, Dug Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Multi point fluorescence measurement system using basic image shifting method and commercial multi mode fiber is presented in this paper. Using a singlet lens, the original fluorescence image of a sample is shifted to another plane which can be monitored using ccd, and at the first image plane independent two fiber tips in an xyz stage deliver each fluorescent signal at a specific sample position to a fluorescence correlation spectroscopy (FCS) with an electron multiplying charge coupled device (EMCCD). The FCS is composed with an EMCCD, which can detect single molecule level fluorescence light. Applying region of interest (ROI) and pixel binning, a time resolution of up to 2 ms can be achieved, which is sufficient to resolve the diffusion of fluorescence micro-sphere in solution. The advantages of implementing EMCCD cameras in wide-field ultra low light imaging, as well as in site-specific multi-point fluorescence measurement system, can consequently also be exploited for spatially and spectrally resolved FCS. Experimental results about FCS with spectrum informations demonstrate the advantage of the simplicity and flexibility of our system. We expect that this multi point measurement system also can be applied to other study of bio molecular dynamics.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Imaging III
Volume7571
DOIs
Publication statusPublished - 2010 May 6
EventSingle Molecule Spectroscopy and Imaging III - San Francisco, CA, United States
Duration: 2010 Jan 232010 Jan 25

Other

OtherSingle Molecule Spectroscopy and Imaging III
CountryUnited States
CitySan Francisco, CA
Period10/1/2310/1/25

Fingerprint

Fluorescence Spectrometry
Fluorescence
Spectroscopy
fluorescence
spectroscopy
Electrons
Equipment and Supplies
charge coupled devices
Light
Charge coupled devices
Molecular Dynamics Simulation
Lenses
electrons
Multimode fibers
fibers
CCD cameras
Molecular dynamics
flexibility
Pixels
pixels

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Song, W., Moon, S., Lee, B. C., Kim, H., Park, C. S., & Kim, D. Y. (2010). Site specific multi-point fluorescence measurement system for fluorescence correlation spectroscopy. In Single Molecule Spectroscopy and Imaging III (Vol. 7571). [757117] https://doi.org/10.1117/12.841468
Song, Woosub ; Moon, Sucbei ; Lee, Byoung Cheol ; Kim, Haedeun ; Park, Chul Seung ; Kim, Dug Young. / Site specific multi-point fluorescence measurement system for fluorescence correlation spectroscopy. Single Molecule Spectroscopy and Imaging III. Vol. 7571 2010.
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Song, W, Moon, S, Lee, BC, Kim, H, Park, CS & Kim, DY 2010, Site specific multi-point fluorescence measurement system for fluorescence correlation spectroscopy. in Single Molecule Spectroscopy and Imaging III. vol. 7571, 757117, Single Molecule Spectroscopy and Imaging III, San Francisco, CA, United States, 10/1/23. https://doi.org/10.1117/12.841468

Site specific multi-point fluorescence measurement system for fluorescence correlation spectroscopy. / Song, Woosub; Moon, Sucbei; Lee, Byoung Cheol; Kim, Haedeun; Park, Chul Seung; Kim, Dug Young.

Single Molecule Spectroscopy and Imaging III. Vol. 7571 2010. 757117.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Song W, Moon S, Lee BC, Kim H, Park CS, Kim DY. Site specific multi-point fluorescence measurement system for fluorescence correlation spectroscopy. In Single Molecule Spectroscopy and Imaging III. Vol. 7571. 2010. 757117 https://doi.org/10.1117/12.841468