Size characterization of liposomes by flow field-flow fractionation and photon correlation spectroscopy: Effect of ionic strength and pH of carrier solutions

Myeong Hee Moon, Ilyong Park, Yonghee Kim

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The effect of ionic strength and pH of carrier solutions on the separation of liposomes by flow field-flow fractionation (flow FFF) has been studied for the determination of accurate vesicle size distribution of liposomes. Retention behaviors of liposomes (PC/PG/cholesterol) are observed in typical buffer solutions (PBS and Tris-HCl) of various ionic strengths as carrier liquids in flow FFF. The average diameters of collected fractions at each flow FFF run are measured by photon correlation spectroscopy (PCS) for the comparison with FFF calculations at corresponding time interval of collected fractions. A reasonable separation of liposomes is observed at I=0.016 M for both buffer solutions. Retention of liposomes is found to be elongated at ionic strengths higher than an optimum condition found experimentally, but it is shortened at a lower ionic strength due to the electrostatic interaction between the channel wall and the liposomes. Finally, size distributions of liposomes are provided comparing the liposome preparations by flow FFF. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)91-100
Number of pages10
JournalJournal of Chromatography A
Volume813
Issue number1
DOIs
Publication statusPublished - 1998 Jul 10

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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