Size characterization of sodium hyaluronate by field programming frit inlet asymmetrical flow field-flow fractionation/multiangle light scattering

Hoonjoo Kim, Heejeong Lee, Myeong Hee Moon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Sodium hyaluronate (NaHA), water soluble polymer having ultra-high molecular weight, is characterized by using on-line frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) and multiangle light scattering (MALS). This study demonstrates the capability of power programming FI-AFlFFF for the separation of NaHA and the applicability of FI-AFlFFF with MALS for the characterization of molecular weight distribution and their structural information. Since sample injection and relaxation in FI-AFlFFF are achieved by using hydrodynamic relaxation, separation of high molecular weight polymers can be achieved smoothly without halting the separation flow. Experiments are carried out with the two different NaHA products (a raw NaHA sample and a thermally degraded NaHA product) and molecular weight distribution and conformations in solution are determined. Influence of sample filtration on the change of molecular weight distribution is also discussed.

Original languageEnglish
Pages (from-to)413-418
Number of pages6
JournalBulletin of the Korean Chemical Society
Volume27
Issue number3
DOIs
Publication statusPublished - 2006 Mar 20

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Inlet flow
Hyaluronic Acid
Fractionation
Light scattering
Flow fields
Molecular weight distribution
Polymers
Molecular weight
Conformations
Hydrodynamics
Water
Experiments

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

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abstract = "Sodium hyaluronate (NaHA), water soluble polymer having ultra-high molecular weight, is characterized by using on-line frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) and multiangle light scattering (MALS). This study demonstrates the capability of power programming FI-AFlFFF for the separation of NaHA and the applicability of FI-AFlFFF with MALS for the characterization of molecular weight distribution and their structural information. Since sample injection and relaxation in FI-AFlFFF are achieved by using hydrodynamic relaxation, separation of high molecular weight polymers can be achieved smoothly without halting the separation flow. Experiments are carried out with the two different NaHA products (a raw NaHA sample and a thermally degraded NaHA product) and molecular weight distribution and conformations in solution are determined. Influence of sample filtration on the change of molecular weight distribution is also discussed.",
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