Skeletal myogenic differentiation of human periodontal ligament stromal cells isolated from orthodontically extracted premolars

Minjung Song, Hana Kim, Yoonjeong Choi, Kyungho Kim, Chooryung Chung

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: To investigate the stem cell-like characteristics of human periodontal ligament (PDL) stromal cells outgrown from orthodontically extracted premolars and to evaluate the potential for myogenic differentiation. Methods: PDL stromal cells were obtained from extracted premolars by using the outgrowth method. Cell morphological features, self-replication capability, and the presence of cell-surface markers, along with osteogenic, adipogenic, and chondrogenic differentiation, were confirmed. In addition, myogenic differentiation was induced by the use of 5-aza-2'-deoxycytidine (5-Aza) for DNA demethylation. Results: PDL stromal cells showed growth patterns and morphological features similar to those of fibroblasts. In contrast, the proliferation rates of premolar PDL stromal cells were similar to those of bone marrow and adipogenic stem cells. PDL stromal cells expressed surface markers of human mesenchymal stem cells (i.e., CD90 and CD105), but not those of hematopoietic stem cells (i.e., CD31 and CD34). PDL stromal cells were differentiated into osteogenic, adipogenic, and chondrogenic lineages. Myotube structures were induced in PDL stromal cells after 5-Aza pretreatment, but not in the absence of 5-Aza pretreatment. Conclusions: PDL stromal cells isolated from extracted premolars can potentially be a good source of postnatal stem cells for oromaxillofacial regeneration in bone and muscle.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalKorean Journal of Orthodontics
Volume42
Issue number5
DOIs
Publication statusPublished - 2012 Nov 19

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Periodontal Ligament
Bicuspid
Stromal Cells
Stem Cells
decitabine
Bone Regeneration
Skeletal Muscle Fibers
Hematopoietic Stem Cells
Mesenchymal Stromal Cells
Fibroblasts
Bone Marrow
Muscles
DNA
Growth

All Science Journal Classification (ASJC) codes

  • Orthodontics

Cite this

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title = "Skeletal myogenic differentiation of human periodontal ligament stromal cells isolated from orthodontically extracted premolars",
abstract = "Objective: To investigate the stem cell-like characteristics of human periodontal ligament (PDL) stromal cells outgrown from orthodontically extracted premolars and to evaluate the potential for myogenic differentiation. Methods: PDL stromal cells were obtained from extracted premolars by using the outgrowth method. Cell morphological features, self-replication capability, and the presence of cell-surface markers, along with osteogenic, adipogenic, and chondrogenic differentiation, were confirmed. In addition, myogenic differentiation was induced by the use of 5-aza-2'-deoxycytidine (5-Aza) for DNA demethylation. Results: PDL stromal cells showed growth patterns and morphological features similar to those of fibroblasts. In contrast, the proliferation rates of premolar PDL stromal cells were similar to those of bone marrow and adipogenic stem cells. PDL stromal cells expressed surface markers of human mesenchymal stem cells (i.e., CD90 and CD105), but not those of hematopoietic stem cells (i.e., CD31 and CD34). PDL stromal cells were differentiated into osteogenic, adipogenic, and chondrogenic lineages. Myotube structures were induced in PDL stromal cells after 5-Aza pretreatment, but not in the absence of 5-Aza pretreatment. Conclusions: PDL stromal cells isolated from extracted premolars can potentially be a good source of postnatal stem cells for oromaxillofacial regeneration in bone and muscle.",
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Skeletal myogenic differentiation of human periodontal ligament stromal cells isolated from orthodontically extracted premolars. / Song, Minjung; Kim, Hana; Choi, Yoonjeong; Kim, Kyungho; Chung, Chooryung.

In: Korean Journal of Orthodontics, Vol. 42, No. 5, 19.11.2012, p. 249-254.

Research output: Contribution to journalArticle

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