Multifunctional magnetic nanoparticles have shown great promise as next-generation imaging and perturbation probes for deciphering molecular and cellular processes. As a consequence of multicomponent integration into a single nanosystem, pre-existing nanoprobes are typically large and show limited access to biological targets present in a crowded microenvironment. Here, we apply organic-phase surface PEGylation, click chemistry, and charge-based valency discrimination principles to develop compact, modular, and monovalent magnetofluorescent nanoparticles (MFNs). We show that MFNs exhibit highly efficient labeling to target receptors present in cells with a dense and thick glycocalyx layer. We use these MFNs to interrogate the E-cadherin-mediated adherens junction formation and F-actin polymerization in a three-dimensional space, demonstrating the utility as modular and versatile mechanogenetic probes in the most demanding single-cell perturbation applications.
Bibliographical noteFunding Information:
This work was supported by IBS-R026-D1 from IBS (J.C. and Y.J.), 1R01GM112081 and 1R01GM126542-01 from the National Institute of General Medical Science (NIGMS) and the National Institute of Health (NIH) (Y.J.), R21CA224364- 01 from the National Cancer Institute (NCI) and the National Institute of Health (NIH) (Y.J.), 1R21NS103240 from the National Institute of Neurological Disorders and Stroke (NINDS) and the NIH (Y.J.), and NRF-2017M3D9A1073494 (Y.H.K.) from the National Research Foundation of Korea (KRF). M.K. is supported by a Life Science Research Foundation Shurl and Kay Curci Foundation fellowship, Burroughs Wellcome Fund, and NRF-2017R1D1A1B03035910.
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics
- Mechanical Engineering