Small-diameter blood vessels engineered with bone marrow-derived cells

Seung Woo Cho, Sang Hyun Lim, Il Kwon Kim, Yoo Sun Hong, Sang Soo Kim, Kyung Jong Yoo, Hyun Young Park, Yangsoo Jang, Byung Chul Chang, Cha Yong Choi, Ki Chul Hwang, Byung Soo Kim

Research output: Contribution to journalArticlepeer-review

223 Citations (Scopus)


Objective: The objective of this study is to investigate if bone marrow-derived cells (BMCs) regenerate vascular tissues and improve patency in tissue-engineered small-diameter (internal diameter = 3 mm) vascular grafts. Summary Background Data: BMCs have demonstrated the ability to differentiate into endothelial-like cells and vascular smooth muscle-like cells and may offer an alternative cell source for vascular tissue engineering. Thus, we tissue-engineered small-diameter vascular grafts with BMCs and decellularized arteries. Methods: Canine BMCs were differentiated in vitro into smooth muscle a-actin/smooth muscle myosin heavy-chain-positive cells and von Willebrand factor/CD31-positive cells and seeded onto decellularized canine carotid arteries (internal diameter = 3 mm). The seeded grafts were implanted in cell donor dogs. The vascular-tissue regeneration and graft patency were investigated with immunohistochemistry and angiography, respectively. Results: The vascular grafts seeded with BMCs remained patent for up to 8 weeks in the canine carotid artery interposition model, whereas nonseeded grafts occluded within 2 weeks. Within 8 weeks after implantation, the vascular grafts showed regeneration of the 3 elements of artery (endothelium, media, and adventitia). BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular grafts, indicating that the BMCs participated in the vascular tissue regeneration. Conclusions: Here we show that BMCs have the potential to regenerate vascular tissues and improve patency in tissue-engineered small-diameter vascular grafts. This is the first report of a small-diameter neovessel engineered with BMCs as a cell source.

Original languageEnglish
Pages (from-to)506-515
Number of pages10
JournalAnnals of surgery
Issue number3
Publication statusPublished - 2005 Mar

All Science Journal Classification (ASJC) codes

  • Surgery


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