Small-diameter blood vessels engineered with bone marrow-derived cells

Seung Woo Cho, Sang Hyun Lim, Il Kwon Kim, Yoo Sun Hong, Sang Soo Kim, Kyung Jong Yoo, Hyun Young Park, Yangsoo Jang, Byung Chul Chang, Cha Yong Choi, Ki Chul Hwang, Byung Soo Kim

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

Objective: The objective of this study is to investigate if bone marrow-derived cells (BMCs) regenerate vascular tissues and improve patency in tissue-engineered small-diameter (internal diameter = 3 mm) vascular grafts. Summary Background Data: BMCs have demonstrated the ability to differentiate into endothelial-like cells and vascular smooth muscle-like cells and may offer an alternative cell source for vascular tissue engineering. Thus, we tissue-engineered small-diameter vascular grafts with BMCs and decellularized arteries. Methods: Canine BMCs were differentiated in vitro into smooth muscle a-actin/smooth muscle myosin heavy-chain-positive cells and von Willebrand factor/CD31-positive cells and seeded onto decellularized canine carotid arteries (internal diameter = 3 mm). The seeded grafts were implanted in cell donor dogs. The vascular-tissue regeneration and graft patency were investigated with immunohistochemistry and angiography, respectively. Results: The vascular grafts seeded with BMCs remained patent for up to 8 weeks in the canine carotid artery interposition model, whereas nonseeded grafts occluded within 2 weeks. Within 8 weeks after implantation, the vascular grafts showed regeneration of the 3 elements of artery (endothelium, media, and adventitia). BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular grafts, indicating that the BMCs participated in the vascular tissue regeneration. Conclusions: Here we show that BMCs have the potential to regenerate vascular tissues and improve patency in tissue-engineered small-diameter vascular grafts. This is the first report of a small-diameter neovessel engineered with BMCs as a cell source.

Original languageEnglish
Pages (from-to)506-515
Number of pages10
JournalAnnals of surgery
Volume241
Issue number3
DOIs
Publication statusPublished - 2005 Mar 1

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Bone Marrow Cells
Blood Vessels
Transplants
Canidae
Regeneration
Arteries
Smooth Muscle Myosins
Adventitia
Myosin Heavy Chains
von Willebrand Factor
Internal Carotid Artery
Tissue Engineering
Fluorescent Dyes
Vascular Smooth Muscle
Carotid Arteries
Smooth Muscle Myocytes
Endothelium
Smooth Muscle
Actins
Angiography

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Cho, S. W., Lim, S. H., Kim, I. K., Hong, Y. S., Kim, S. S., Yoo, K. J., ... Kim, B. S. (2005). Small-diameter blood vessels engineered with bone marrow-derived cells. Annals of surgery, 241(3), 506-515. https://doi.org/10.1097/01.sla.0000154268.12239.ed
Cho, Seung Woo ; Lim, Sang Hyun ; Kim, Il Kwon ; Hong, Yoo Sun ; Kim, Sang Soo ; Yoo, Kyung Jong ; Park, Hyun Young ; Jang, Yangsoo ; Chang, Byung Chul ; Choi, Cha Yong ; Hwang, Ki Chul ; Kim, Byung Soo. / Small-diameter blood vessels engineered with bone marrow-derived cells. In: Annals of surgery. 2005 ; Vol. 241, No. 3. pp. 506-515.
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abstract = "Objective: The objective of this study is to investigate if bone marrow-derived cells (BMCs) regenerate vascular tissues and improve patency in tissue-engineered small-diameter (internal diameter = 3 mm) vascular grafts. Summary Background Data: BMCs have demonstrated the ability to differentiate into endothelial-like cells and vascular smooth muscle-like cells and may offer an alternative cell source for vascular tissue engineering. Thus, we tissue-engineered small-diameter vascular grafts with BMCs and decellularized arteries. Methods: Canine BMCs were differentiated in vitro into smooth muscle a-actin/smooth muscle myosin heavy-chain-positive cells and von Willebrand factor/CD31-positive cells and seeded onto decellularized canine carotid arteries (internal diameter = 3 mm). The seeded grafts were implanted in cell donor dogs. The vascular-tissue regeneration and graft patency were investigated with immunohistochemistry and angiography, respectively. Results: The vascular grafts seeded with BMCs remained patent for up to 8 weeks in the canine carotid artery interposition model, whereas nonseeded grafts occluded within 2 weeks. Within 8 weeks after implantation, the vascular grafts showed regeneration of the 3 elements of artery (endothelium, media, and adventitia). BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular grafts, indicating that the BMCs participated in the vascular tissue regeneration. Conclusions: Here we show that BMCs have the potential to regenerate vascular tissues and improve patency in tissue-engineered small-diameter vascular grafts. This is the first report of a small-diameter neovessel engineered with BMCs as a cell source.",
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Cho, SW, Lim, SH, Kim, IK, Hong, YS, Kim, SS, Yoo, KJ, Park, HY, Jang, Y, Chang, BC, Choi, CY, Hwang, KC & Kim, BS 2005, 'Small-diameter blood vessels engineered with bone marrow-derived cells', Annals of surgery, vol. 241, no. 3, pp. 506-515. https://doi.org/10.1097/01.sla.0000154268.12239.ed

Small-diameter blood vessels engineered with bone marrow-derived cells. / Cho, Seung Woo; Lim, Sang Hyun; Kim, Il Kwon; Hong, Yoo Sun; Kim, Sang Soo; Yoo, Kyung Jong; Park, Hyun Young; Jang, Yangsoo; Chang, Byung Chul; Choi, Cha Yong; Hwang, Ki Chul; Kim, Byung Soo.

In: Annals of surgery, Vol. 241, No. 3, 01.03.2005, p. 506-515.

Research output: Contribution to journalArticle

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T1 - Small-diameter blood vessels engineered with bone marrow-derived cells

AU - Cho, Seung Woo

AU - Lim, Sang Hyun

AU - Kim, Il Kwon

AU - Hong, Yoo Sun

AU - Kim, Sang Soo

AU - Yoo, Kyung Jong

AU - Park, Hyun Young

AU - Jang, Yangsoo

AU - Chang, Byung Chul

AU - Choi, Cha Yong

AU - Hwang, Ki Chul

AU - Kim, Byung Soo

PY - 2005/3/1

Y1 - 2005/3/1

N2 - Objective: The objective of this study is to investigate if bone marrow-derived cells (BMCs) regenerate vascular tissues and improve patency in tissue-engineered small-diameter (internal diameter = 3 mm) vascular grafts. Summary Background Data: BMCs have demonstrated the ability to differentiate into endothelial-like cells and vascular smooth muscle-like cells and may offer an alternative cell source for vascular tissue engineering. Thus, we tissue-engineered small-diameter vascular grafts with BMCs and decellularized arteries. Methods: Canine BMCs were differentiated in vitro into smooth muscle a-actin/smooth muscle myosin heavy-chain-positive cells and von Willebrand factor/CD31-positive cells and seeded onto decellularized canine carotid arteries (internal diameter = 3 mm). The seeded grafts were implanted in cell donor dogs. The vascular-tissue regeneration and graft patency were investigated with immunohistochemistry and angiography, respectively. Results: The vascular grafts seeded with BMCs remained patent for up to 8 weeks in the canine carotid artery interposition model, whereas nonseeded grafts occluded within 2 weeks. Within 8 weeks after implantation, the vascular grafts showed regeneration of the 3 elements of artery (endothelium, media, and adventitia). BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular grafts, indicating that the BMCs participated in the vascular tissue regeneration. Conclusions: Here we show that BMCs have the potential to regenerate vascular tissues and improve patency in tissue-engineered small-diameter vascular grafts. This is the first report of a small-diameter neovessel engineered with BMCs as a cell source.

AB - Objective: The objective of this study is to investigate if bone marrow-derived cells (BMCs) regenerate vascular tissues and improve patency in tissue-engineered small-diameter (internal diameter = 3 mm) vascular grafts. Summary Background Data: BMCs have demonstrated the ability to differentiate into endothelial-like cells and vascular smooth muscle-like cells and may offer an alternative cell source for vascular tissue engineering. Thus, we tissue-engineered small-diameter vascular grafts with BMCs and decellularized arteries. Methods: Canine BMCs were differentiated in vitro into smooth muscle a-actin/smooth muscle myosin heavy-chain-positive cells and von Willebrand factor/CD31-positive cells and seeded onto decellularized canine carotid arteries (internal diameter = 3 mm). The seeded grafts were implanted in cell donor dogs. The vascular-tissue regeneration and graft patency were investigated with immunohistochemistry and angiography, respectively. Results: The vascular grafts seeded with BMCs remained patent for up to 8 weeks in the canine carotid artery interposition model, whereas nonseeded grafts occluded within 2 weeks. Within 8 weeks after implantation, the vascular grafts showed regeneration of the 3 elements of artery (endothelium, media, and adventitia). BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular grafts, indicating that the BMCs participated in the vascular tissue regeneration. Conclusions: Here we show that BMCs have the potential to regenerate vascular tissues and improve patency in tissue-engineered small-diameter vascular grafts. This is the first report of a small-diameter neovessel engineered with BMCs as a cell source.

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