Smartphone-based multi-contrast microscope using color-multiplexed illumination

Daeseong Jung, Jun Ho Choi, Soocheol Kim, Suho Ryu, Wonchan Lee, Jong Seok Lee, Chulmin Joo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We present a portable multi-contrast microscope capable of producing bright-field, dark-field, and differential phase contrast images of thin biological specimens on a smartphone platform. The microscopy method is based on an imaging scheme termed "color-coded light-emitting-diode (LED) microscopy (cLEDscope)," in which a specimen is illuminated with a color-coded LED array and light transmitted through the specimen is recorded by a color image sensor. Decomposition of the image into red, green, and blue colors and subsequent computation enable multi-contrast imaging in a single shot. In order to transform a smartphone into a multi-contrast imaging device, we developed an add-on module composed of a patterned color micro-LED array, specimen stage, and miniature objective. Simple installation of this module onto a smartphone enables multi-contrast imaging of transparent specimens. In addition, an Android-based app was implemented to acquire an image, perform the associated computation, and display the multi-contrast images in real time. Herein, the details of our smartphone module and experimental demonstrations with various biological specimens are presented.

Original languageEnglish
Article number7564
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

All Science Journal Classification (ASJC) codes

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