Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells

Hyunsoo Shin, Yeo Song Lee, Yongchan Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Epigenetic mechanisms of histone acetylation/deacetylation play an important role in the regulation of gene expression associated with the cell cycle and apoptosis. Recently, sodium butyrate, a histone deacetylase (HDAC) inhibitor, has been shown to exhibit anticancer effects via differentiation and apoptosis of cancer cells. Sodium butyrate may be a potential anticancer chemotherapeutic drug; however, the precise mechanism underlying the anticancer effects of sodium butyrate has not been clearly elucidated. In the present study, we investigated the role of death-associated protein kinase (DAPK) on the apoptosis of human gastric cancer cells induced by sodium butyrate. We observed that sodium butyrate induced apoptosis in human gastric cancer cells. Treatment with the HDAC inhibitor sodium butyrate increased the expression of caspase-3 and DAPK1/2 genes but decreased the expression of Bcl-2 in human gastric cancer cells. The expression of DAPK3, p53 and p21 were not altered by sodium butyrate treatment. Analysis of the general expression patterns revealed that sodium butyrate increased the expression of DAPK1/2 but decreased the expression of FAK and induced changes in the proliferation of apoptosis-related genes in human gastric cancer cells. These data suggest that DAPK expression prompts apoptosis by reducing the FAK protein level in sodium butyrate-induced apoptosis of human gastric cancer cells.

Original languageEnglish
Pages (from-to)1111-1115
Number of pages5
JournalOncology Reports
Volume27
Issue number4
DOIs
Publication statusPublished - 2012 Apr 1

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Death-Associated Protein Kinases
Butyric Acid
Stomach Neoplasms
Apoptosis
Histone Deacetylase Inhibitors
Gene Expression Regulation
Acetylation
Epigenomics
Caspase 3
Antineoplastic Agents
Histones
Cell Cycle

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Shin, Hyunsoo ; Lee, Yeo Song ; Lee, Yongchan. / Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells. In: Oncology Reports. 2012 ; Vol. 27, No. 4. pp. 1111-1115.
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Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells. / Shin, Hyunsoo; Lee, Yeo Song; Lee, Yongchan.

In: Oncology Reports, Vol. 27, No. 4, 01.04.2012, p. 1111-1115.

Research output: Contribution to journalArticle

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