Sol-gel-derived thick-film conductometric biosensor for urea determination in serum

Wonyong Lee, Seung Ryeol Kim, Tae Han Kim, Kang Shin Lee, Min Chol Shin, Je Kyun Park

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

A conductometric urea biosensor based on sol-gel-immobilized urease on a screen-printed inter-digitated array (IDA) electrode has been developed. The screen-printed thick-film IDA electrode has proven to be an excellent conductometric transducer in which the admittance signal was dominated by the conductance signal and the resulting dynamic range was wide enough to be employed for the construction of conductometric urea biosensor. Urease was immobilized by a conventional sol-gel process using tetramethyl orthosilicate as a precursor. The sol-gel-derived urea biosensor showed a reasonably wide linear dynamic range 0.03-2.5mM in 5.0mM imidazole-HCl buffer at pH 7.5 (detection limit=30μM, signal:noise=2), thus enabling it to be used to monitor urea in a diluted serum sample with the differential measurement format consisting of an active IDA electrode with sol-gel-immobilized urease and a control IDA electrode with sol-gel-immobilized bovine serum albumin. The urea biosensor exhibited good sensor-to-sensor reproducibility (4.4%) and storage stability (63% of its original activity retained after 25 days). The coupling of the sol-gel immobilization method and screen-printing technology can offer mass production of a durable urea biosensor. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)195-203
Number of pages9
JournalAnalytica Chimica Acta
Volume404
Issue number2
DOIs
Publication statusPublished - 2000 Jan 17

Fingerprint

Biosensing Techniques
Polymethyl Methacrylate
Thick films
Biosensors
urea
Sol-gels
Urea
serum
gel
Gels
Urease
Electrodes
electrode
Serum
orthosilicate
sensor
Printing
Screen printing
Sensors
transducer

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Lee, Wonyong ; Kim, Seung Ryeol ; Kim, Tae Han ; Lee, Kang Shin ; Shin, Min Chol ; Park, Je Kyun. / Sol-gel-derived thick-film conductometric biosensor for urea determination in serum. In: Analytica Chimica Acta. 2000 ; Vol. 404, No. 2. pp. 195-203.
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Sol-gel-derived thick-film conductometric biosensor for urea determination in serum. / Lee, Wonyong; Kim, Seung Ryeol; Kim, Tae Han; Lee, Kang Shin; Shin, Min Chol; Park, Je Kyun.

In: Analytica Chimica Acta, Vol. 404, No. 2, 17.01.2000, p. 195-203.

Research output: Contribution to journalArticle

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