Abstract
Rapid, real-time detection of pathogenic microorganisms is an emerging and quickly evolving field of research, especially with regard to microorganisms that pose a major threat to public health. Herein, a new method that uses bioimpedance and solid culture medium for the real-time monitoring of bacterial growth is introduced. We fabricated a new impedimetric biosensor by integrating solid medium and two plane electrodes attached on two facing sides of an acryl well. During bioelectrical impedance analysis, the solid medium showed the characteristics of a homogenous conductive material. In a real-time impedance measurement, our solid medium biosensor could monitor bacterial growth in situ with a detection time of ∼4 h. We applied different culture media (e.g. Tryptic Soy Agar, Luria Agar, and Nutrient Agar) to our biosensor and found that a low conductive medium was optimal for the monitoring of the bacterial growth due to low threshold in the variation of the impedance signal. Our data indicate that the solid medium biosensor is useful for detection of pathogenic microorganisms, thereby providing a new analytical tool for impedance microbiology.
| Original language | English |
|---|---|
| Pages (from-to) | 357-362 |
| Number of pages | 6 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 137 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 Mar 28 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry
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Solid-medium-integrated impedimetric biosensor for real-time monitoring of microorganisms. / Choi, Ahmi; Park, Jae Sung; Jung, Hyo il.
In: Sensors and Actuators, B: Chemical, Vol. 137, No. 1, 28.03.2009, p. 357-362.Research output: Contribution to journal › Article
TY - JOUR
T1 - Solid-medium-integrated impedimetric biosensor for real-time monitoring of microorganisms
AU - Choi, Ahmi
AU - Park, Jae Sung
AU - Jung, Hyo il
PY - 2009/3/28
Y1 - 2009/3/28
N2 - Rapid, real-time detection of pathogenic microorganisms is an emerging and quickly evolving field of research, especially with regard to microorganisms that pose a major threat to public health. Herein, a new method that uses bioimpedance and solid culture medium for the real-time monitoring of bacterial growth is introduced. We fabricated a new impedimetric biosensor by integrating solid medium and two plane electrodes attached on two facing sides of an acryl well. During bioelectrical impedance analysis, the solid medium showed the characteristics of a homogenous conductive material. In a real-time impedance measurement, our solid medium biosensor could monitor bacterial growth in situ with a detection time of ∼4 h. We applied different culture media (e.g. Tryptic Soy Agar, Luria Agar, and Nutrient Agar) to our biosensor and found that a low conductive medium was optimal for the monitoring of the bacterial growth due to low threshold in the variation of the impedance signal. Our data indicate that the solid medium biosensor is useful for detection of pathogenic microorganisms, thereby providing a new analytical tool for impedance microbiology.
AB - Rapid, real-time detection of pathogenic microorganisms is an emerging and quickly evolving field of research, especially with regard to microorganisms that pose a major threat to public health. Herein, a new method that uses bioimpedance and solid culture medium for the real-time monitoring of bacterial growth is introduced. We fabricated a new impedimetric biosensor by integrating solid medium and two plane electrodes attached on two facing sides of an acryl well. During bioelectrical impedance analysis, the solid medium showed the characteristics of a homogenous conductive material. In a real-time impedance measurement, our solid medium biosensor could monitor bacterial growth in situ with a detection time of ∼4 h. We applied different culture media (e.g. Tryptic Soy Agar, Luria Agar, and Nutrient Agar) to our biosensor and found that a low conductive medium was optimal for the monitoring of the bacterial growth due to low threshold in the variation of the impedance signal. Our data indicate that the solid medium biosensor is useful for detection of pathogenic microorganisms, thereby providing a new analytical tool for impedance microbiology.
UR - http://www.scopus.com/inward/record.url?scp=60549102644&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=60549102644&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2008.12.062
DO - 10.1016/j.snb.2008.12.062
M3 - Article
VL - 137
SP - 357
EP - 362
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 1
ER -