Solitary fibrous tumor/hemangiopericytoma metastasizes extracranially, associated with altered expression of wnt5a and mmp9

Jong Hwan Hong; Myung Giun Noh; Md Rashedunnabi Akanda; Yeong Jin Kim; Se Hoon Kim; Tae Young Jung; Shin Jung; Jae Hyuk Lee; Joon Haeng Rhee; Kyung Keun Kim; Sung Sun Kim; Kyung Hwa Lee; Kyung Sub Moon

doi:10.3390/cancers13051142

Solitary fibrous tumor/hemangiopericytoma metastasizes extracranially, associated with altered expression of wnt5a and mmp9

Jong Hwan Hong, Myung Giun Noh, Md Rashedunnabi Akanda, Yeong Jin Kim, Se Hoon Kim, Tae Young Jung, Shin Jung, Jae Hyuk Lee, Joon Haeng Rhee, Kyung Keun Kim, Sung Sun Kim, Kyung Hwa Lee, Kyung Sub Moon

Department of Pathology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Solitary fibrous tumor/hemangiopericytoma (SFT/HPC) is a mesenchymal tumor originat-ing from various soft tissues and meninges, which carries the NAB2-STAT6 fusion gene. Meningeal/intracranial SFT/HPCs (icSFT/HPC) have a poor clinical outcome with metastatic behav-ior compared to soft tissue/extracranial SFT/HPCs (exSFT/HPC), but the underlying genetic factors are unclear. Differentially expressed genes (DEGs) were analyzed by NanoString nCounter assay using RNA extracted from formalin-fixed paraffin-embedded (FFPE) tissue samples. Additionally, immunohistochemistry (IHC) was performed on 32 cases of exSFT/HPC, 18 cases of icSFT/HPC, and additional recurrent or metastatic cases to verify the findings. Pathway analysis revealed that the WNT signaling pathway was enriched in exSFT/HPC. Analysis of DEGs showed that expression of WNT5A was lower and that of MMP9 was higher in icSFT/HPC than in exSFT/HPC (p = 0.008 and p = 0.035, respectively). IHC showed that WNT5A and CD34 expression was high in exSFT/HPC (p < 0.001, both), while that of MMP9 was high in icSFT/HPC (p = 0.001). Expression of CLDN5 in tumoral vessels was locally decreased in icSFT/HPC (p < 0.001). The results suggested that decreased WNT5A expression, together with increased MMP9 expression, in icSFT/HPC, may affect vascular tightness and prompt tumor cells to metastasize extracranially.

Original language	English
Article number	1142
Pages (from-to)	1-15
Number of pages	15
Journal	Cancers
Volume	13
Issue number	5
DOIs	https://doi.org/10.3390/cancers13051142
Publication status	Published - 2021 Mar 1

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Minist (2020M3A9G3080281, 2019R1A2B5B01070598, and 2018R1A5A2024181 for K.-H.L., 2020R1C1C1007832 for K.-S.M.) and by Chonnam National University Hospital Biomedical Resear.ch Institute (HCRI19030 for K.-S.M. and K.-H.L.). There was no role of the funding bodies in the design of the study, in the collection, analysis, and interpretation of data or in writing the manuscript.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/cancers13051142

Cite this

@article{626d6222f0f249e484b4a41ce48ade65,

title = "Solitary fibrous tumor/hemangiopericytoma metastasizes extracranially, associated with altered expression of wnt5a and mmp9",

abstract = "Solitary fibrous tumor/hemangiopericytoma (SFT/HPC) is a mesenchymal tumor originat-ing from various soft tissues and meninges, which carries the NAB2-STAT6 fusion gene. Meningeal/intracranial SFT/HPCs (icSFT/HPC) have a poor clinical outcome with metastatic behav-ior compared to soft tissue/extracranial SFT/HPCs (exSFT/HPC), but the underlying genetic factors are unclear. Differentially expressed genes (DEGs) were analyzed by NanoString nCounter assay using RNA extracted from formalin-fixed paraffin-embedded (FFPE) tissue samples. Additionally, immunohistochemistry (IHC) was performed on 32 cases of exSFT/HPC, 18 cases of icSFT/HPC, and additional recurrent or metastatic cases to verify the findings. Pathway analysis revealed that the WNT signaling pathway was enriched in exSFT/HPC. Analysis of DEGs showed that expression of WNT5A was lower and that of MMP9 was higher in icSFT/HPC than in exSFT/HPC (p = 0.008 and p = 0.035, respectively). IHC showed that WNT5A and CD34 expression was high in exSFT/HPC (p < 0.001, both), while that of MMP9 was high in icSFT/HPC (p = 0.001). Expression of CLDN5 in tumoral vessels was locally decreased in icSFT/HPC (p < 0.001). The results suggested that decreased WNT5A expression, together with increased MMP9 expression, in icSFT/HPC, may affect vascular tightness and prompt tumor cells to metastasize extracranially.",

author = "Hong, {Jong Hwan} and Noh, {Myung Giun} and Akanda, {Md Rashedunnabi} and Kim, {Yeong Jin} and Kim, {Se Hoon} and Jung, {Tae Young} and Shin Jung and Lee, {Jae Hyuk} and Rhee, {Joon Haeng} and Kim, {Kyung Keun} and Kim, {Sung Sun} and Lee, {Kyung Hwa} and Moon, {Kyung Sub}",

note = "Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Minist (2020M3A9G3080281, 2019R1A2B5B01070598, and 2018R1A5A2024181 for K.-H.L., 2020R1C1C1007832 for K.-S.M.) and by Chonnam National University Hospital Biomedical Resear.ch Institute (HCRI19030 for K.-S.M. and K.-H.L.). There was no role of the funding bodies in the design of the study, in the collection, analysis, and interpretation of data or in writing the manuscript. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = mar,

day = "1",

doi = "10.3390/cancers13051142",

language = "English",

volume = "13",

pages = "1--15",

journal = "Cancers",

issn = "2072-6694",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "5",

}

TY - JOUR

T1 - Solitary fibrous tumor/hemangiopericytoma metastasizes extracranially, associated with altered expression of wnt5a and mmp9

AU - Hong, Jong Hwan

AU - Noh, Myung Giun

AU - Akanda, Md Rashedunnabi

AU - Kim, Yeong Jin

AU - Kim, Se Hoon

AU - Jung, Tae Young

AU - Jung, Shin

AU - Lee, Jae Hyuk

AU - Rhee, Joon Haeng

AU - Kim, Kyung Keun

AU - Kim, Sung Sun

AU - Lee, Kyung Hwa

AU - Moon, Kyung Sub

N1 - Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Minist (2020M3A9G3080281, 2019R1A2B5B01070598, and 2018R1A5A2024181 for K.-H.L., 2020R1C1C1007832 for K.-S.M.) and by Chonnam National University Hospital Biomedical Resear.ch Institute (HCRI19030 for K.-S.M. and K.-H.L.). There was no role of the funding bodies in the design of the study, in the collection, analysis, and interpretation of data or in writing the manuscript. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/3/1

Y1 - 2021/3/1

N2 - Solitary fibrous tumor/hemangiopericytoma (SFT/HPC) is a mesenchymal tumor originat-ing from various soft tissues and meninges, which carries the NAB2-STAT6 fusion gene. Meningeal/intracranial SFT/HPCs (icSFT/HPC) have a poor clinical outcome with metastatic behav-ior compared to soft tissue/extracranial SFT/HPCs (exSFT/HPC), but the underlying genetic factors are unclear. Differentially expressed genes (DEGs) were analyzed by NanoString nCounter assay using RNA extracted from formalin-fixed paraffin-embedded (FFPE) tissue samples. Additionally, immunohistochemistry (IHC) was performed on 32 cases of exSFT/HPC, 18 cases of icSFT/HPC, and additional recurrent or metastatic cases to verify the findings. Pathway analysis revealed that the WNT signaling pathway was enriched in exSFT/HPC. Analysis of DEGs showed that expression of WNT5A was lower and that of MMP9 was higher in icSFT/HPC than in exSFT/HPC (p = 0.008 and p = 0.035, respectively). IHC showed that WNT5A and CD34 expression was high in exSFT/HPC (p < 0.001, both), while that of MMP9 was high in icSFT/HPC (p = 0.001). Expression of CLDN5 in tumoral vessels was locally decreased in icSFT/HPC (p < 0.001). The results suggested that decreased WNT5A expression, together with increased MMP9 expression, in icSFT/HPC, may affect vascular tightness and prompt tumor cells to metastasize extracranially.

AB - Solitary fibrous tumor/hemangiopericytoma (SFT/HPC) is a mesenchymal tumor originat-ing from various soft tissues and meninges, which carries the NAB2-STAT6 fusion gene. Meningeal/intracranial SFT/HPCs (icSFT/HPC) have a poor clinical outcome with metastatic behav-ior compared to soft tissue/extracranial SFT/HPCs (exSFT/HPC), but the underlying genetic factors are unclear. Differentially expressed genes (DEGs) were analyzed by NanoString nCounter assay using RNA extracted from formalin-fixed paraffin-embedded (FFPE) tissue samples. Additionally, immunohistochemistry (IHC) was performed on 32 cases of exSFT/HPC, 18 cases of icSFT/HPC, and additional recurrent or metastatic cases to verify the findings. Pathway analysis revealed that the WNT signaling pathway was enriched in exSFT/HPC. Analysis of DEGs showed that expression of WNT5A was lower and that of MMP9 was higher in icSFT/HPC than in exSFT/HPC (p = 0.008 and p = 0.035, respectively). IHC showed that WNT5A and CD34 expression was high in exSFT/HPC (p < 0.001, both), while that of MMP9 was high in icSFT/HPC (p = 0.001). Expression of CLDN5 in tumoral vessels was locally decreased in icSFT/HPC (p < 0.001). The results suggested that decreased WNT5A expression, together with increased MMP9 expression, in icSFT/HPC, may affect vascular tightness and prompt tumor cells to metastasize extracranially.

UR - http://www.scopus.com/inward/record.url?scp=85101998091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85101998091&partnerID=8YFLogxK

U2 - 10.3390/cancers13051142

DO - 10.3390/cancers13051142

M3 - Article

AN - SCOPUS:85101998091

SN - 2072-6694

VL - 13

SP - 1

EP - 15

JO - Cancers

JF - Cancers

IS - 5

M1 - 1142

ER -

Solitary fibrous tumor/hemangiopericytoma metastasizes extracranially, associated with altered expression of wnt5a and mmp9

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this