Soluble HLA-G generated by proteolytic shedding inhibits NK-mediated cell lysis

Gyu Man Park, Sunray Lee, Boyoun Park, Eunkyung Kim, Jinwook Shin, Kwangmin Cho, Kwangseog Ahn

Research output: Contribution to journalArticlepeer-review

138 Citations (Scopus)

Abstract

In contrast to the classical HLA class Ia molecules, the nonclassical HLA-G primary transcript is alternatively spliced to generate several mRNAs that encode four membrane-bound and three soluble isoforms. This study demonstrated that the soluble form of HLA-G can also be generated by metalloproteinase-dependent shedding at post-translational level. These soluble HLA-G1 molecules generated by the cleavage of membrane-bound HLA-G1 associate with β2-microglobulin and contain bound peptides that are stable at physiological conditions. This report further showed that the soluble HLA-G1 is able to protect HLA class I-negative K562 cells from NK lysis, suggesting that soluble HLA-G could act as an immunoregulator in NK cell recognition and possibly in other immune responses.

Original languageEnglish
Pages (from-to)606-611
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume313
Issue number3
DOIs
Publication statusPublished - 2004 Jan 16

Bibliographical note

Funding Information:
The authors thank Drs. Daniel Geraghty and Michael McMaster for providing valuable reagents and the LG-Yeonam Cultural Foundation for the assistance. This work was supported by grants from the Molecular and Cellular BioDiscovery Research Program (M10106000054) and the Center for Functional Analysis of Human Genome (FG0003010011) of the Ministry of Science and Technology.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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