Solution conformations of the substrates and inhibitor of hepatitis C virus NS3 protease

Jung Hoon Lee, Keunsu Bang, Jin Won Jung, In Ae Ahn, Seonggu Ro, Weontae Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) has been known to be an enveloped virus with a positive strand RNA genome and the major agent of the vast majority of transfusion associated cases of hepatitis. For viral replication, HCV structural proteins are first processed by host cell signal peptidases and NS2/NS3 site of the nonstructural protein is cleaved by a zinc-dependent protease NS2 with N-terminal NS3. The four remaining junctions are cleaved by a separate NS3 protease. The solution conformations of NS4B/5A, NS5A/5B substrates and NS5A/5B inhibitor have been determined by two-dimensional nuclear magnetic resonance (NMR) spectroscopy. NMR data suggested that the both NS5A/5B substrate and inhibitor appeared to have a folded turn-like conformation not only between P1 and P6 position but also C-terminal region, whereas the NS4B/5A substrate exhibited mostly extended conformation. In addition, we have found that the conformation of the NS5A/5B inhibitor slightly differs from that of NS5A/5B substrate peptide, suggesting different binding mode for NS3 protease. These findings will be of importance for designing efficient inhibitor to suppress HCV processing.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalBulletin of the Korean Chemical Society
Volume20
Issue number3
Publication statusPublished - 1999 Mar 20

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Conformations
Viruses
Peptide Hydrolases
Substrates
Viral Structural Proteins
Nuclear magnetic resonance spectroscopy
Zinc
Genes
Nuclear magnetic resonance
RNA
Peptides
hepatitis C virus NS3 protein
Processing
Proteins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Lee, Jung Hoon ; Bang, Keunsu ; Jung, Jin Won ; Ahn, In Ae ; Ro, Seonggu ; Lee, Weontae. / Solution conformations of the substrates and inhibitor of hepatitis C virus NS3 protease. In: Bulletin of the Korean Chemical Society. 1999 ; Vol. 20, No. 3. pp. 301-306.
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Solution conformations of the substrates and inhibitor of hepatitis C virus NS3 protease. / Lee, Jung Hoon; Bang, Keunsu; Jung, Jin Won; Ahn, In Ae; Ro, Seonggu; Lee, Weontae.

In: Bulletin of the Korean Chemical Society, Vol. 20, No. 3, 20.03.1999, p. 301-306.

Research output: Contribution to journalArticle

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