Solution Structure and Rpn1 Interaction of the UBL Domain of Human RNA Polymerase II C-Terminal Domain Phosphatase

Ji Hye Yun, Sunggeon Ko, Chung Kyung Lee, Hae Kap Cheong, Chaejoon Cheong, Jong Bok Yoon, Weontae Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The ubiquitin-like modifier (UBL) domain of ubiquitin-like domain proteins (UDPs) interacts specifically with subunits of the 26 S proteasome. A novel UDP, ubiquitin-like domain-containing C-terminal domain phosphatase (UBLCP1), has been identified as an interacting partner of the 26 S proteasome. We determined the high-resolution solution structure of the UBL domain of human UBLCP1 by nuclear magnetic resonance spectroscopy. The UBL domain of hUBLCP1 has a unique β-strand (β3) and β3-α2 loop, instead of the canonical β4 observed in other UBL domains. The molecular topology and secondary structures are different from those of known UBL domains including that of fly UBLCP1. Data from backbone dynamics shows that the β3-α2 loop is relatively rigid although it might have intrinsic dynamic profile. The positively charged residues of the β3-α2 loop are involved in interacting with the C-terminal leucine-rich repeat-like domain of Rpn1.

Original languageEnglish
Article numbere62981
JournalPloS one
Volume8
Issue number5
DOIs
Publication statusPublished - 2013 May 7

Fingerprint

RNA Polymerase III
RNA Polymerase II
DNA-directed RNA polymerase
ubiquitin
Ubiquitin
Phosphoric Monoester Hydrolases
Ubiquitins
Proteasome Endopeptidase Complex
proteasome endopeptidase complex
Leucine
Diptera
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance spectroscopy
topology
leucine
nuclear magnetic resonance spectroscopy
Topology
proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Yun, Ji Hye ; Ko, Sunggeon ; Lee, Chung Kyung ; Cheong, Hae Kap ; Cheong, Chaejoon ; Yoon, Jong Bok ; Lee, Weontae. / Solution Structure and Rpn1 Interaction of the UBL Domain of Human RNA Polymerase II C-Terminal Domain Phosphatase. In: PloS one. 2013 ; Vol. 8, No. 5.
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Solution Structure and Rpn1 Interaction of the UBL Domain of Human RNA Polymerase II C-Terminal Domain Phosphatase. / Yun, Ji Hye; Ko, Sunggeon; Lee, Chung Kyung; Cheong, Hae Kap; Cheong, Chaejoon; Yoon, Jong Bok; Lee, Weontae.

In: PloS one, Vol. 8, No. 5, e62981, 07.05.2013.

Research output: Contribution to journalArticle

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