Solution structure of a novel calcium binding protein, MTH1880, from Methanobacterium thermoautotrophicum

Chang Hun Lee, Jin Won Jung, Adelinda Yee, Cheryl H. Arrowsmith, Weontae Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

MTH1880 is a hypothetical protein from Methanobacterium thermoautotrophicum, a target organism of structural genomics. The solution structure determined by NMR spectroscopy demonstrates a typical α + β-fold found in many proteins with different functions. The molecular surface of the protein reveals a small, highly acidic pocket comprising loop B (Asp36, Asp37, Asp38), the end of β2 (Glu39), and loop D (Ser57, Ser58, Ser61), indicating that the protein would have a possible cation binding site. The NMR resonances of several amino acids within the acidic binding pocket in MTH1880, shifted upon addition of calcium ion. This calcium binding motif and overall topology of MTH1880 differ from those of other calcium binding proteins. MTH1880 did not show a calcium-induced conformational change typical of calcium sensor proteins. Therefore, we propose that the MTH1880 protein contains a novel motif for calcium-specific binding, and may function as a calcium buffering protein.

Original languageEnglish
Pages (from-to)1148-1154
Number of pages7
JournalProtein Science
Volume13
Issue number4
DOIs
Publication statusPublished - 2004 Apr 1

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Methanobacterium
Calcium-Binding Proteins
Calcium
Proteins
Acidic Amino Acids
Genomics
Nuclear magnetic resonance spectroscopy
Cations
Membrane Proteins
Magnetic Resonance Spectroscopy
Binding Sites
Nuclear magnetic resonance
Topology
Ions
Amino Acids
Sensors

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Lee, Chang Hun ; Jung, Jin Won ; Yee, Adelinda ; Arrowsmith, Cheryl H. ; Lee, Weontae. / Solution structure of a novel calcium binding protein, MTH1880, from Methanobacterium thermoautotrophicum. In: Protein Science. 2004 ; Vol. 13, No. 4. pp. 1148-1154.
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Solution structure of a novel calcium binding protein, MTH1880, from Methanobacterium thermoautotrophicum. / Lee, Chang Hun; Jung, Jin Won; Yee, Adelinda; Arrowsmith, Cheryl H.; Lee, Weontae.

In: Protein Science, Vol. 13, No. 4, 01.04.2004, p. 1148-1154.

Research output: Contribution to journalArticle

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