Solution structure of telomere binding domain of AtTRB2 derived from Arabidopsis thaliana

Ji Hye Yun, Won Kyung Lee, Heeyoun Kim, Eunhee Kim, Chaejoon Cheong, Myeon Haeng Cho, Weontae Lee

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Telomere homeostasis is regulated by telomere-associated proteins, and the Myb domain is well conserved for telomere binding. AtTRB2 is a member of the SMH (Single-Myb-Histone)-like family in Arabidopsis thaliana, having an N-terminal Myb domain, which is responsible for DNA binding. The Myb domain of AtTRB2 contains three α-helices and loops for DNA binding, which is unusual given that other plant telomere-binding proteins have an additional fourth helix that is essential for DNA binding. To understand the structural role for telomeric DNA binding of AtTRB2, we determined the solution structure of the Myb domain of AtTRB2 (AtTRB21-64) using nuclear magnetic resonance (NMR) spectroscopy. In addition, the inter-molecular interaction between AtTRB21-64 and telomeric DNA has been characterized by the electrophoretic mobility shift assay (EMSA) and NMR titration analyses for both plant (TTTAGGG)n and human (TTAGGG)n telomere sequences. Data revealed that Trp28, Arg29, and Val47 residues located in Helix 2 and Helix 3 are crucial for DNA binding, which are well conserved among other plant telomere binding proteins. We concluded that although AtTRB2 is devoid of the additional fourth helix in the Myb-extension domain, it is able to bind to plant telomeric repeat sequences as well as human telomeric repeat sequences.

Original languageEnglish
Pages (from-to)436-442
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume452
Issue number3
DOIs
Publication statusPublished - 2014 Sep 26

Bibliographical note

Funding Information:
This work was supported by Mid-career Researcher Program ( NRF-2013R1A2A2A01068963 ) and supported in part by Basic Science Research Program (No. NRF-2013 R1A12009383 to M.H.C.) through NRF grant funded by the MEST . J.H. Yun and W.K. Lee are recipients of Brain Korea 21 graduate student scholarship.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Solution structure of telomere binding domain of AtTRB2 derived from Arabidopsis thaliana'. Together they form a unique fingerprint.

Cite this