Solution structure of the cytoplasmic domain of syndecan-3 by two-dimensional NMR spectroscopy

Young Yeo In, Bonkyung Koo, Eok Soo Oh, Inn Oc Han, Weon Tae Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Syndecan-3 is a cell-surface heparan sulfate proteoglycan, which performs a variety of functions during cell adhension process. It is also a coreceptor for growth factor, mediating cell-cell and cell-matrix interaction. Syndecan-3 contains a cytoplasmic domain potentially associated with the cytoskeleton. Syndecan-3 is specifically expressed in neuron cell and has related to neuron cell differentiation and development of actin filament in cell migration. Syndecans each have a unique, central, and variable (V) region in their cytoplasmic domains. And that region of syndecan-3 may modulate the interactions of the conserved C1 regions of the cytoplasmic domains by tyrosine phosphorylation. Cytoplasmic domain of syndecan-3 has been synthesized for NMR structural studies. The solution structure of syndecan-3 cytoplasmic domain has been determined by two-dimensional NMR spectroscopy and simulated-annealing calculation. The cytoplasmic domain of the syndecan proteins has a tendency to form a dimmer conformation with a central cavity, however, that of syndecan-3 demonstrated a monomer conformation with a flexible region near C-terminus. The structural information might add knowledge about the structure-function relationships among syndecan proteins.

Original languageEnglish
Pages (from-to)1013-1017
Number of pages5
JournalBulletin of the Korean Chemical Society
Volume29
Issue number5
DOIs
Publication statusPublished - 2008 May 20

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Syndecan-3
Nuclear magnetic resonance spectroscopy
Syndecans
Neurons
Conformations
Heparan Sulfate Proteoglycans
Phosphorylation
Simulated annealing
Tyrosine
Actins
Intercellular Signaling Peptides and Proteins
Proteins
Monomers
Nuclear magnetic resonance

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

In, Young Yeo ; Koo, Bonkyung ; Oh, Eok Soo ; Han, Inn Oc ; Lee, Weon Tae. / Solution structure of the cytoplasmic domain of syndecan-3 by two-dimensional NMR spectroscopy. In: Bulletin of the Korean Chemical Society. 2008 ; Vol. 29, No. 5. pp. 1013-1017.
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Solution structure of the cytoplasmic domain of syndecan-3 by two-dimensional NMR spectroscopy. / In, Young Yeo; Koo, Bonkyung; Oh, Eok Soo; Han, Inn Oc; Lee, Weon Tae.

In: Bulletin of the Korean Chemical Society, Vol. 29, No. 5, 20.05.2008, p. 1013-1017.

Research output: Contribution to journalArticle

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